ArticleStem cell research & therapy2016
Human endothelial and foetal femur-derived stem cell co-cultures modulate osteogenesis and angiogenesis.
Article in Stem cell research & therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
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- Three-Dimensional Printing/Bioprinting and Cellular Therapies for Regenerative Medicine: Current Advances.Journal of functional biomaterials · 2025Review
- Use of stem cells in bone regeneration in cleft palate patients: review and recommendations.Journal of the Korean Association of Oral and Maxillofacial Surgeons · 2022Review
- Human Periodontal Ligament Stem Cell and Umbilical Vein Endothelial Cell Co-Culture to Prevascularize Scaffolds for Angiogenic and Osteogenic Tissue Engineering.International journal of molecular sciences · 2021Article
- The Duo of Osteogenic and Angiogenic Differentiation in ADSC-Derived Spheroids.Frontiers in cell and developmental biology · 2021Article
- 3D Bioprinting of Vascularized Tissues forFrontiers in bioengineering and biotechnology · 2021Review
- In vivo delivery of VEGF RNA and protein to increase osteogenesis and intraosseous angiogenesis.Scientific reports · 2019Article
- [Heterotopic osteogenesis study of tissue engineered bone by co-culture of vascular endothelial cells and adipose-derived stem cells].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2019Article
- 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Bioprinting in Vascularization StrategiesIranian biomedical journal · 2019Review
- Efficacy of intraoperatively prepared cell-based constructs for bone regeneration.Stem cell research & therapy · 2018Article
- Evolving applications of the egg: chorioallantoic membrane assay andJournal of tissue engineeringReview
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5 authors at 1 institution in 1 country.
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Abstract
backgroundA dynamic vasculature is a prerequisite for bone formation where the interaction of bone cells and endothelial cells is essential for both the development and the healing process of bone. Enhanced understanding of the specific mediators involved in bone cell and endothelial cell interactions offers new avenues for skeletal regenerative applications. This study has investigated the osteogenic and angiogenic potential of co-cultures of human foetal diaphyseal or epiphyseal cells with human umbilical vein endothelial cells (HUVEC) in the presence and absence of vascular endothelial growth factor (VEGF) supplementation.
methodsEarly osteogenic activities of the co-cultures (± VEGF) were assessed by alkaline phosphatase (ALP) activity. Osteogenic and angiogenic gene expression was measured using quantitative polymerase chain reaction. An ex vivo organotypic embryonic chick (E11) femur culture model was used to determine the osteogenic effects of VEGF as determined using micro-computed tomography (μCT) and Alcian blue/Sirius red histochemistry and immunocytochemistry for expression of CD31.
resultsALP activity and gene expression of ALP and Type-1 collagen was enhanced in foetal skeletal/HUVECs co-cultures. In foetal diaphyseal/HUVECs co-cultures, VEGF reduced the levels of ALP activity and displayed a negligible effect on von Willebrand factor (vWF) and VEGF gene expression. In contrast, VEGF supplementation was observed to significantly increase FLT-1 and KDR gene expression in co-cultures with modulation of expression enhanced, compared to VEGF skeletal monocultures. In the organotypic chick model, addition of VEGF significantly enhanced bone formation, which coincided with elevated levels of CD31-positive cells in the mid-diaphyseal region of the femurs.
conclusionThese studies demonstrate a differential skeletal response of early foetal skeletal cells, when co-cultured with endothelial cells and the potential of co-culture models for bone repair. The differential effect of VEGF supplementation on markers of angiogenesis and osteogenesis in co-cultures and organ cultures, demonstrate the importance of the intricate temporal coordination of osteogenic and angiogenic processes during bone formation and implications therein for effective approaches to bone regenerative therapies.
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