Trial reportJournal of cellular and molecular medicine2016
Phosphatidylserine externalization and procoagulant activation of erythrocytes induced by Pseudomonas aeruginosa virulence factor pyocyanin.
Trial report in Journal of cellular and molecular medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 81 citations in OpenAlex.
- Apoptosis and eryptosis: similarities and differences.Apoptosis : an international journal on programmed cell death · 2024Review
- Olive Oil Phenols Prevent Mercury-Induced Phosphatidylserine Exposure and Morphological Changes in Human Erythrocytes Regardless of Their Different Scavenging Activity.International journal of molecular sciences · 2022Article
- Anti-Eryptotic Activity of Food-Derived Phytochemicals and Natural Compounds.International journal of molecular sciences · 2022Review
- Review
- Eryptosis as a New Insight in Malaria Pathogenesis.Frontiers in immunology · 2022Review
- Metabolic Influences Modulating Erythrocyte Deformability and Eryptosis.Metabolites · 2021Review
- Trp-Containing Antibacterial Peptides Impair Quorum Sensing and Biofilm Development in Multidrug-ResistantFrontiers in microbiology · 2021Article
- Clostridium perfringens epsilon toxin binds to erythrocyte MAL receptors and triggers phosphatidylserine exposure.Journal of cellular and molecular medicine · 2020Article
- Identification of a novel RhlI/R-PrrH-LasI/Phzc/PhzD signalling cascade and its implication inEmerging microbes & infections · 2019Article
- Correlative Light-Electron Microscopy detects lipopolysaccharide and its association with fibrin fibres in Parkinson's Disease, Alzheimer's Disease and Type 2 Diabetes Mellitus.Scientific reports · 2018Article
- No effects without causes: the Iron Dysregulation and Dormant Microbes hypothesis for chronic, inflammatory diseases.Biological reviews of the Cambridge Philosophical Society · 2018Review
- Role of extracellular vesicles in the development of sepsis-induced coagulopathy.Journal of intensive care · 2018Review
- The Effect of Sepsis on the Erythrocyte.International journal of molecular sciences · 2017Review
- The stabilizing effect of an oligomeric proanthocyanidin on red blood cell membrane structure of poorly controlled Type II diabetes.Nutrition & diabetes · 2017Article
- Effects of IL-1β, IL-6 and IL-8 on erythrocytes, platelets and clot viscoelasticity.Scientific reports · 2016Article
- A Bacterial Component to Alzheimer's-Type Dementia Seen via a Systems Biology Approach that Links Iron Dysregulation and Inflammagen Shedding to Disease.Journal of Alzheimer's disease : JAD · 2016Review
- Pseudomonas aeruginosa Microcolonies in Coronary Thrombi from Patients with ST-Segment Elevation Myocardial Infarction.PloS one · 2016Article
- A Dormant Microbial Component in the Development of Preeclampsia.Frontiers in medicine · 2016Review
- Erythrocyte degradation, metabolism, secretion, and communication with immune cells in the blood during sepsis: A review.Tzu chi medical journalReview
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The opportunistic pathogen Pseudomonas aeruginosa causes a wide range of infections in multiple hosts by releasing an arsenal of virulence factors such as pyocyanin. Despite numerous reports on the pleiotropic cellular targets of pyocyanin toxicity in vivo, its impact on erythrocytes remains elusive. Erythrocytes undergo an apoptosis-like cell death called eryptosis which is characterized by cell shrinkage and phosphatidylserine (PS) externalization; this process confers a procoagulant phenotype on erythrocytes as well as fosters their phagocytosis and subsequent clearance from the circulation. Herein, we demonstrate that P. aeruginosa pyocyanin-elicited PS exposure and cell shrinkage in erythrocyte while preserving the membrane integrity. Mechanistically, exposure of erythrocytes to pyocyanin showed increased cytosolic Ca(2+) activity as well as Ca(2+) -dependent proteolytic processing of μ-calpain. Pyocyanin further up-regulated erythrocyte ceramide abundance and triggered the production of reactive oxygen species. Pyocyanin-induced increased PS externalization in erythrocytes translated into enhanced prothrombin activation and fibrin generation in plasma. As judged by carboxyfluorescein succinimidyl-ester labelling, pyocyanin-treated erythrocytes were cleared faster from the murine circulation as compared to untreated erythrocytes. Furthermore, erythrocytes incubated in plasma from patients with P. aeruginosa sepsis showed increased PS exposure as compared to erythrocytes incubated in plasma from healthy donors. In conclusion, the present study discloses the eryptosis-inducing effect of the virulence factor pyocyanin, thereby shedding light on a potentially important mechanism in the systemic complications of P. aeruginosa infection.
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