ArticleCellular and molecular life sciences : CMLS2016
The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix.
Article in Cellular and molecular life sciences : CMLS, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Microfluidic Interrogation of Chitin-Induced Calcium Oscillations in the MossPlants (Basel, Switzerland) · 2026Article
- Understanding of Plant Salt Tolerance Mechanisms and Application to Molecular Breeding.International journal of molecular sciences · 2024Review
- The Plant Vacuole as Heterologous System to Characterize the Functional Properties of TPC Channels.Handbook of experimental pharmacology · 2023Article
- Grapevine (Vitis vinifera) responses to salt stress and alkali stress: transcriptional and metabolic profiling.BMC plant biology · 2022Article
- Major vacuolar TPC1 channel in stress signaling: what matters, KStress biology · 2022Review
- Voltage-gating and cytosolic CaProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Computational Analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) Permeation Pathway.International journal of molecular sciences · 2021Article
- Endolysosomal CaCancers · 2018Review
- Phosphatidylinositol-3,5-bisphosphate lipid-binding-induced activation of the human two-pore channel 2.Cellular and molecular life sciences : CMLS · 2018Article
- Structural basis for activation of voltage sensor domains in an ion channel TPC1.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Structural Model of the mIgM B-Cell Receptor Transmembrane Domain From Self-Association Molecular Dynamics Simulations.Frontiers in immunology · 2018Article
- On the structure and mechanism of two-pore channels.The FEBS journal · 2018Article
- Two-pore channels (TPCs): Novel voltage-gated ion channels with pleiotropic functions.Channels (Austin, Tex.) · 2017Review
- Exploring the Formation and the Structure of Synaptobrevin Oligomers in a Model Membrane.Biophysical journal · 2016Article
- New Insights on Plant Salt Tolerance Mechanisms and Their Potential Use for Breeding.Frontiers in plant science · 2016Review
- Membrane-Mediated Oligomerization of G Protein Coupled Receptors and Its Implications for GPCR Function.Frontiers in physiology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-pore channels (TPCs) constitute a family of intracellular cation channels with diverse permeation properties and functions in animals and plants. In the model plant Arabidopsis, the vacuolar cation channel TPC1 is involved in propagation of calcium waves and in cation homeostasis. Here, we discovered that the dimerization of a predicted helix within the carboxyl-terminus (CTH) is essential for the activity of TPC1. Bimolecular fluorescence complementation and co-immunoprecipitation demonstrated the interaction of the two C-termini and pointed towards the involvement of the CTH in this process. Synthetic CTH peptides dimerized with a dissociation constant of 3.9 µM. Disruption of this domain in TPC1 either by deletion or point mutations impeded the dimerization and cation transport. The homo-dimerization of the CTH was analyzed in silico using coarse-grained molecular dynamics (MD) simulations for the study of aggregation, followed by atomistic MD simulations. The simulations revealed that the helical region of the wild type, but not a mutated CTH forms a highly stable, antiparallel dimer with characteristics of a coiled-coil. We propose that the voltage- and Ca(2+)-sensitive conformation of TPC1 depends on C-terminal dimerization, adding an additional layer to the complex regulation of two-pore cation channels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.