Evidence map›Paper›PMID 26780865›Full record

ArticleCytotherapy2016

Chromosomal stability of mesenchymal stromal cells during in vitro culture.

Brian G Stultz, Kathleen McGinnis, Elaine E Thompson, Jessica L Lo Surdo, Steven R Bauer, Deborah A Hursh

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytotherapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
6.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 80 citations in OpenAlex.

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  14. Isolation and characterization of feline dental pulp stem cells.Journal of feline medicine and surgery · 2023
    Article
  15. Article
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  17. Amniotic fluid characteristics and its application in stem cell therapy: A review.International journal of reproductive biomedicine · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Brian G StultzDivision of Cell and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Kathleen McGinnisDivision of Cell and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Elaine E ThompsonDivision of Cell and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Jessica L Lo SurdoDivision of Cell and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Steven R BauerDivision of Cell and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Deborah A HurshDivision of Cell and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA. Electronic address: deborah.hursh@fda.hhs.gov.
Center for Biologics Evaluation and Research · US

Funding

Intramural FDA HHS FD999999
6 · The paper itself

Abstract

BACKGROUND

aimsMesenchymal stromal cells (MSCs) are being investigated for use in cell therapy. The extensive in vitro expansion necessary to obtain sufficient cells for clinical use increases the risk that genetically abnormal cells will arise and be propagated during cell culture. Genetic abnormalities may lead to transformation and poor performance in clinical use, and are a critical safety concern for cell therapies using MSCs.

methodsWe used spectral karyotyping (SKY) to investigate the genetic stability of human MSCs from ten donors during passaging.

resultsOur data indicate that chromosomal abnormalities exist in MSCs at early passages and can be clonally propagated. The karyotypic abnormalities observed during our study diminished during passage.

conclusionsKaryotyping of MSCs reveals characteristics which may be valuable in deciding the suitability of cells for further use. Karyotypic analysis is useful for monitoring the genetic stability of MSCs during expansion.

Indexed as

Cell Culture TechniquesAdultBone Marrow CellsCell- and Tissue-Based TherapyCells, CulturedChromosomal InstabilityChromosome AberrationsClone CellsFemaleHumansKaryotypingMaleMesenchymal Stem CellsYoung Adultgenomic stabilitymesenchymal stromal cellsMSCSKYspectral karyotyping

Identifiers

PMID26780865
PMCPMC5516473
OpenAlexW2262298819

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.