ArticleNature genetics2016
Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins.
Article in Nature genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 54 citations in OpenAlex.
- Unraveling the role of PIWI/piRNAs in stem cell therapy: Epigenetic mechanisms and therapeutic potentials.Genes & diseases · 2026Review
- TheGenes · 2026Article
- Article
- Binding of heterochromatin protein Rhino to a subset of piRNA clusters depends on a combination of two histone marks.Nature structural & molecular biology · 2025Article
- Integration of multi-omics data to unveil the molecular landscape and role of piRNAs in early-onset colorectal cancer.BMC medicine · 2025Article
- PIWI proteins and piRNAs: key regulators of stem cell biology.Frontiers in cell and developmental biology · 2025Review
- Advances in PIWI-piRNA function in female reproduction in mammals.Acta biochimica et biophysica Sinica · 2024Review
- Selective degradation of PL2L60 by metabolic stresses‑induced autophagy suppresses multi‑cancer growth.Oncology reports · 2024Article
- Exosomal small RNA profiling in first-trimester maternal blood explores early molecular pathways of preterm preeclampsia.Frontiers in immunology · 2024Article
- Article
- cDNA Cloning of Feline PIWIL1 and Evaluation of Expression in the Testis of the Domestic Cat.International journal of molecular sciences · 2023Article
- Review
- Hexavalent chromium-induced epigenetic instability and transposon activation lead to phenotypic variations and tumors inEnvironmental epigenetics · 2023Article
- Stonewall prevents expression of ectopic genes in the ovary and accumulates at insulator elements in D. melanogaster.PLoS genetics · 2022Article
- Epigenetic regulation of drosophila germline stem cell maintenance and differentiation.Developmental biology · 2021Review
- PIWIL1 governs the crosstalk of cancer cell metabolism and immunosuppressive microenvironment in hepatocellular carcinoma.Signal transduction and targeted therapy · 2021Article
- Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries.G3 (Bethesda, Md.) · 2021Article
- Piwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression.Cell reports · 2021Article
- Nuclear architecture as an intrinsic regulator of Drosophila female germline stem cell maintenance.Current opinion in insect science · 2020Review
- Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
The Drosophila melanogaster Piwi protein regulates both niche and intrinsic mechanisms to maintain germline stem cells, but its underlying mechanism remains unclear. Here we report that Piwi interacts with Polycomb group complexes PRC1 and PRC2 in niche and germline cells to regulate ovarian germline stem cells and oogenesis. Piwi physically interacts with the PRC2 subunits Su(z)12 and Esc in the ovary and in vitro. Chromatin coimmunoprecipitation of Piwi, the PRC2 enzymatic subunit E(z), histone H3 trimethylated at lysine 27 (H3K27me3) and RNA polymerase II in wild-type and piwi mutant ovaries demonstrates that Piwi binds a conserved DNA motif at ∼ 72 genomic sites and inhibits PRC2 binding to many non-Piwi-binding genomic targets and H3K27 trimethylation. Moreover, Piwi influences RNA polymerase II activities in Drosophila ovaries, likely via inhibiting PRC2. We hypothesize that Piwi negatively regulates PRC2 binding by sequestering PRC2 in the nucleoplasm, thus reducing PRC2 binding to many targets and influencing transcription during oogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.