ArticleJournal of visualized experiments : JoVE2016
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 81 citations in OpenAlex.
- NADPH oxidase 3 inhibition preserves hearing in mice after stereotactic radiosurgery.Molecular therapy. Nucleic acids · 2026Article
- Blood-labyrinth barrier disruption associates with hypercholesterolemia-induced hearing loss.iScience · 2026Article
- Neuroprotective potential of a TrkB-FL-derived cell-penetrating peptide in cochlear synaptopathy and noise-induced hearing loss.Molecular medicine (Cambridge, Mass.) · 2026Article
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- Sphingolipid-neutralizing molecular therapy reduces psychosine cytotoxicity in Krabbe disease.iScience · 2026Article
- Electromotility can be disassociated from gating charge movement in outer hair cells of conditional alpha2 spectrin knockout mice.The Journal of biological chemistry · 2026Article
- The aryl hydrocarbon receptor is expressed in non-sensory cells in the cochlea and regulates hair cell survival.Frontiers in molecular neuroscience · 2026Article
- Introducing a translationally relevant mouse model of radiosurgery-induced unilateral hearing loss.Frontiers in neuroscience · 2026Article
- Protocol for assessing auditory function, cochlear synaptopathy, and afferent terminal morphology in laboratory mice.STAR protocols · 2025Article
- Adipose Tissue Dysfunction Induced by High-Fat Diet Consumption Is Associated with Higher Otoacoustic Emissions Threshold in Mice C57BL/6.Nutrients · 2025Article
- Cryosectioning and Immunostaining Mouse Inner Ear Tissue: From Embryonic to Adult Stages.Journal of visualized experiments : JoVE · 2025Article
- The Auditory Brainstem Response Diagnoses Alzheimer-Like Disease in the 5xFAD Mouse Model.eNeuro · 2025Article
- Rational design of a Lfng-enhancer AAV construct drives specific and efficient gene expression in inner ear supporting cells.Hearing research · 2025Article
- Mutations in unfolded protein response regulator ATF6 cause hearing and vision loss syndrome.The Journal of clinical investigation · 2025Article
- Morphological phenotyping of the aging cochlea in inbred C57BL/6N and outbred CD1 mouse strains.Aging cell · 2025Article
- Genetic and pharmacologic alterations of claudin9 levels suffice to induce functional and mature inner hair cells.bioRxiv : the preprint server for biology · 2024Article
- TUBB4B is essential for the cytoskeletal architecture of cochlear supporting cells and motile cilia development.Communications biology · 2024Article
- Demyelination and NaeNeuro · 2024Article
- The transcription factorFrontiers in cellular neuroscience · 2024Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The organ of Corti, housed in the cochlea of the inner ear, contains mechanosensory hair cells and surrounding supporting cells which are organized in a spiral shape and have a tonotopic gradient for sound detection. The mouse cochlea is approximately 6 mm long and often divided into three turns (apex, middle, and base) for analysis. To investigate cell loss, cell division, or mosaic gene expression, the whole mount or surface preparation of the cochlea is useful. This dissection method allows visualization of all cells within the organ of Corti when combined with immunostaining and confocal microscopy to image cells at different planes in the z-axis. Multiple optical cross-sections can also be obtained from these z-stack images. In addition, the whole mount dissection method can be used for scanning electron microscopy, although a different fixation method is needed. Here, we present a method to isolate the organ of Corti as three intact cochlear turns (apex, middle, and base). This method can be used for mice ranging from one week of age through adulthood and differs from the technique used for neonatal samples where calcification of the cochlea is incomplete. A slightly modified version can be used for dissection of the rat cochlea. We also demonstrate a procedure for immunostaining with fluorescently tagged antibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.