Evidence map›Paper›PMID 26779524›Full record

ArticleGlobal cardiology science & practice2015

Serum and tissue biomarkers in aortic stenosis.

Alkistis Kapelouzou, Loukas Tsourelis, Loukas Kaklamanis, Dimitrios Degiannis, Nektarios Kogerakis, Dennis V Cokkinos

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Global cardiology science & practice, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 48 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Alkistis KapelouzouCenter of Clinical, Experimental Surgery, & Translation Research. Biomedical Research Foundation Academy of Athens (BRFAA), Soranou Efesiou 4 11527Athens, Greece.
Loukas TsourelisDepartment of Pathology, Onassis Cardiac Surgery Center, Avenue Sygrou 356 17674Athens, Greece.
Loukas KaklamanisDepartment of Pathology, Onassis Cardiac Surgery Center, Avenue Sygrou 356 17674Athens, Greece.
Dimitrios DegiannisLaboratory of Molecular Immunopathology and Istocompatibility Onassis Cardiac Surgery Center, Avenue Sygrou 356 17674Athens, Greece.
Nektarios KogerakisDepartment of Pathology, Onassis Cardiac Surgery Center, Avenue Sygrou 356 17674Athens, Greece.
Dennis V CokkinosCenter of Clinical, Experimental Surgery, & Translation Research. Biomedical Research Foundation Academy of Athens (BRFAA), Soranou Efesiou 4 11527Athens, Greece.
Onassis Cardiac Surgery Center · GRAcademy of Athens · GRBiomedical Research Foundation of the Academy of Athens · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCalcific aortic valve stenosis (CAVS) is seen in a large proportion of individuals over 60 years. It is an active process, influenced by lipid accumulation, mechanical stress, inflammation, and abnormal extracellular matrix turnover. Various biomarkers (BMs) are studied, as regards mechanisms, diagnosis and prognosis.

methodsIn the calcified valves calcium deposition, elastin fragmentation and disorganization of cellular matrix were assessed, together with expression of OPN, OPG, osteocalcin (OCN) and RL2. We prospectively studied the following serum BMs in 60 patients with CAVS and compared them to 20 healthy controls, free from any cardiac disease: Matrix metalloproteinases (MMP) 2 and 9 and tissue inhibitor of metalloproteinase 1 (TIMP1), which regulate collagen turnover, inflammatory factors, i.e. tumor necrosis factor a (TNFa), interleukin 2 (IL2), transforming growth factor β1 (TGF-β1) which regulates fibrosis, fetuin-A (fet-A), osteopontin (OPN), osteoprotegerin (OPG), sclerostin (SOST), and relaxin-2 (RL2) which positively or negatively regulate calcification. Monocyte chemoattractant protein 1 (MCP-1) which regulates migration and infiltration of monocytes/macrophages was also studied as well as malondialdehyde (MDA) an oxidative marker.

resultsExtent of tissue valve calcification (Alizarin Red stain) was negatively correlated with tissue elastin, and RL2, and positively correlated with tissue OCN and serum TIMP1 and MCP-1 and negatively with MMP9. Tissue OCN was positively correlated with OPN and negatively with the elastin. Tissue OPN was negatively correlated with elastin and OPG. Tissue OPN OPG and RL2 were not correlated with serum levels In the serum we found in patients statistically lower TIMP1, fet-A and RL2 levels, while all other BMs were higher compared to the healthy group. Positive correlations between SOST and IL2, OPG and MDA but negative with TNFa and OPN were found; also MMP9 was negatively correlated with TNFa and MCP-1 was negatively correlated with TIMP1.

conclusionWe found that many BMs expressing calcification, collagen breakdown, or formation, and inflammation are increased in the valve tissue and in the serum of patients with CAVS as compared with healthy group. Our findings may give new insights towards diagnosis but also therapy. Thus antisclerostin, and antiflammatory agents could be tried for preventing aortic calcification progression.

Indexed as

aortic valve stenosisbiomarkerscalcificationrelaxin-2Sclerostin

Identifiers

PMID26779524
PMCPMC4710866
OpenAlexW2251812278

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.