ArticleFrontiers in cellular neuroscience2015
In vivo Calcium Imaging of Evoked Calcium Waves in the Embryonic Cortex.
Article in Frontiers in cellular neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Para-uterine imaging protocol: Stabilizing living mouse embryos within the maternal abdomen for in vivo imaging and visually guided physiology.STAR protocols · 2026Article
- A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis.Journal of neuroinflammation · 2025Article
- Development of spontaneous and sensory evoked network activity in rodent cerebral cortexFrontiers in cellular neuroscience · 2025Review
- Calcium Signaling during Cortical Apical Dendrite Initiation: A Role for Cajal-Retzius Neurons.International journal of molecular sciences · 2023Article
- Cortical activity emerges in region-specific patterns during early brain development.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Cortical activity emerges in region-specific patterns during early brain development.bioRxiv : the preprint server for biology · 2023Article
- Orchestration of Ion Channels and Transporters in Neocortical Development and Neurological Disorders.Frontiers in neuroscience · 2022Review
- Inflammation of the Embryonic Choroid Plexus Barrier following Maternal Immune Activation.Developmental cell · 2020Article
- Intravital imaging of mouse embryos.Science (New York, N.Y.) · 2020Article
- Activation of the phagocyte NADPH oxidase/NOX2 and myeloperoxidase in the mouse brain during pilocarpine-induced temporal lobe epilepsy and inhibition by ketamine.Inflammopharmacology · 2020Article
- Two-photon microscopic observation of cell-production dynamics in the developing mammalian neocortex in utero.Development, growth & differentiation · 2020Article
- Leukocyte Trafficking and Hemostasis in the Mouse FetusFrontiers in cell and developmental biology · 2020Article
- How Do Electric Fields Coordinate Neuronal Migration and Maturation in the Developing Cortex?Frontiers in cell and developmental biology · 2020Review
- In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity.Scientific reports · 2018Article
- Three-photon imaging of mouse brain structure and function through the intact skull.Nature methods · 2018Article
- Clock Scan Protocol for Image Analysis: ImageJ Plugins.Journal of visualized experiments : JoVE · 2017Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dynamics of intracellular calcium fluxes are instrumental in the proliferation, differentiation, and migration of neuronal cells. Knowledge thus far of the relationship between these calcium changes and physiological processes in the developing brain has derived principally from ex vivo and in vitro experiments. Here, we present a new method to image intracellular calcium flux in the cerebral cortex of live rodent embryos, whilst attached to the dam through the umbilical cord. Using this approach we demonstrate induction of calcium waves by laser stimulation. These waves are sensitive to ATP-receptor blockade and are significantly increased by pharmacological facilitation of intracellular-calcium release. This approach is the closest to physiological conditions yet achieved for imaging of calcium in the embryonic brain and as such opens new avenues for the study of prenatal brain development. Furthermore, the developed method could open the possibilities of preclinical translational studies in embryos particularly important for developmentally related diseases such as schizophrenia and autism.
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