ArticleRedox biology2016
Lipidomics and H2(18)O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos.
Article in Redox biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 36 citations in OpenAlex.
- Lipid Remodeling in Mouse SR-B1-Deficient Embryos with Oxidative Stress-Associated Neural Tube Defects.Antioxidants (Basel, Switzerland) · 2026Article
- Deciphering the enigma of the function of alpha-tocopherol as a vitamin.Free radical biology & medicine · 2024Article
- Creation of a novel zebrafish model with low DHA status to study the role of maternal nutrition during neurodevelopment.bioRxiv : the preprint server for biology · 2024Article
- Palmitoleic acid as a coordinating molecule between the invasive pinewood nematode and its newly associated fungi.The ISME journal · 2023Article
- Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle.Antioxidants (Basel, Switzerland) · 2023Article
- Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio).The Journal of nutritional biochemistry · 2021Article
- Dose-Dependent Pulmonary Toxicity of Aerosolized Vitamin E Acetate.American journal of respiratory cell and molecular biology · 2020Article
- Ecotoxico-lipidomics: An emerging concept to understand chemical-metabolic relationships in comparative fish models.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2020Review
- Vitamin E is necessary for zebrafish nervous system development.Scientific reports · 2020Article
- Targeting the Liver-Brain Axis with Hop-Derived Flavonoids Improves Lipid Metabolism and Cognitive Performance in Mice.Molecular nutrition & food research · 2020Article
- Regulation of lipid peroxidation and ferroptosis in diverse species.Genes & development · 2018Review
- Chronic vitamin E deficiency impairs cognitive function in adult zebrafish via dysregulation of brain lipids and energy metabolism.Free radical biology & medicine · 2017Article
- Vitamin E deficiency during embryogenesis in zebrafish causes lasting metabolic and cognitive impairments despite refeeding adequate diets.Free radical biology & medicine · 2017Article
- Lethal dysregulation of energy metabolism during embryonic vitamin E deficiency.Free radical biology & medicine · 2017Article
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6 authors at 1 institution in 1 country.
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Abstract
We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without (E-) or with added α-tocopherol (E+, 500mg RRR-α-tocopheryl acetate/kg diet) for a minimum of 80 days, and then spawned them to obtain E- and E+ embryos. The E- compared with E+ embryos were 82% less responsive (p<0.01) to a light/dark stimulus at 96h post-fertilization (hpf), demonstrating impaired locomotor behavior, even in the absence of gross morphological defects. Evaluation of phospholipid (PL) and lysophospholipid (lyso-PL) composition using untargeted lipidomics in E- compared with E+ embryos at 24, 48, 72, and 120hpf showed that four PLs and three lyso-PLs containing docosahexaenoic acid (DHA), including lysophosphatidylcholine (LPC 22:6, required for transport of DHA into the brain, p<0.001), were at lower concentrations in E- at all time-points. Additionally, H2(18)O labeling experiments revealed enhanced turnover of LPC 22:6 (p<0.001) and three other DHA-containing PLs in the E- compared with the E+ embryos, suggesting that increased membrane remodeling is a result of PL depletion. Together, these data indicate that α-tocopherol deficiency in the zebrafish embryo causes the specific depletion and increased turnover of DHA-containing PL and lyso-PLs, which may compromise DHA delivery to the brain and thereby contribute to the functional impairments observed in E- embryos.
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