ArticleBiomaterials2016
In vivo tissue has non-linear rheological behavior distinct from 3D biomimetic hydrogels, as determined by AMOTIV microscopy.
Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Guidance for 3D traction force microscopy today and in the next decade.Nature methods · 2026Review
- Macrophages Mediate Mesoscale Brain Mechanical Homeostasis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Measuring age-dependent viscoelasticity of organelles, cells and organisms with time-shared optical tweezer microrheology.Nature nanotechnology · 2025Article
- Macrophage mediated mesoscale brain mechanical homeostasis mechanically imaged via optical tweezers and Brillouin microscopybioRxiv : the preprint server for biology · 2024Article
- YAP localization mediates mechanical adaptation of human cancer cells during extravasationbioRxiv : the preprint server for biology · 2023Article
- From cells to form: A roadmap to study shape emergence in vivo.Biophysical journal · 2023Review
- Vascular regulation of disseminated tumor cells during metastatic spread.Biophysics reviews · 2023Review
- Cell-specific cargo delivery using synthetic bacterial spores.Cell reports · 2023Article
- Article
- Multimodal microscale mechanical mapping of cancer cells in complex microenvironments.Biophysical journal · 2022Article
- Nanoscale Tracking Combined with Cell-Scale Microrheology Reveals Stepwise Increases in Force Generated by Cancer Cell Protrusions.Nano letters · 2022Article
- Cell mediated remodeling of stiffness matched collagen and fibrin scaffolds.Scientific reports · 2022Article
- Patterned photocrosslinking to establish stiffness anisotropies in fibrous 3D hydrogels.Acta biomaterialia · 2022Article
- Multi-frequency passive and active microrheology with optical tweezers.Scientific reports · 2021Article
- Magnetic probe-based microrheology reveals local softening and stiffening of 3D collagen matrices by fibroblasts.Biomedical microdevices · 2021Article
- Dynamic actin cross-linking governs the cytoplasm's transition to fluid-like behavior.Molecular biology of the cell · 2020Article
- Integrated Optofluidic Chip for Oscillatory Microrheology.Scientific reports · 2020Article
- Quantifying the Local Mechanical Properties of Cells in a Fibrous Three-Dimensional Microenvironment.Biophysical journal · 2019Article
- Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish.Cell systems · 2019Article
- High-frequency microrheology in 3D reveals mismatch between cytoskeletal and extracellular matrix mechanics.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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Authors and funding
4 authors.
Funding
Abstract
Variation in matrix elasticity has been shown to determine cell fate in both differentiation and development of malignant phenotype. The tissue microenvironment provides complex biochemical and biophysical signals in part due to the architectural heterogeneities found in extracellular matrices (ECMs). Three dimensional cell cultures can partially mimic in vivo tissue architecture, but to truly understand the role of viscoelasticity on cell fate, we must first determine in vivo tissue mechanical properties to improve in vitro models. We employed Active Microrheology by Optical Trapping InVivo (AMOTIV), using in situ calibration to measure in vivo zebrafish tissue mechanics. Previously used trap calibration methods overestimate complex moduli by ∼ 2-20 fold compared to AMOTIV. Applying differential microscale stresses and strains showed that hyaluronic acid (HA) gels display semi-flexible polymer behavior, while laminin-rich ECM hydrogels display flexible polymer behavior. In contrast, zebrafish tissues displayed different moduli at different stresses, with higher power law exponents at lower stresses, indicating that living tissue has greater stress dependence than the 3D hydrogels examined. To our knowledge, this work is the first vertebrate tissue rheological characterization performed in vivo. Our fundamental observations are important for the development and refinement of in vitro platforms.
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