Evidence map›Paper›PMID 26772433›Full record

ArticleNeuroscience2016

MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain.

H Singh, M Li, L Hall, S Chen, S Sukur, R Lu, A Caputo, A L Meredith, E Stefani, L Toro

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 48 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. CaInternational journal of molecular sciences · 2023
    Review
  8. Review
  9. Mitochondrial ion channels in cardiac function.American journal of physiology. Cell physiology · 2021
    Review
  10. Review
  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. BKCells · 2019
    Article
  17. Cell Cycle Regulation by CaInternational journal of molecular sciences · 2018
    Article
  18. Inhibition of BKPhysiological reports · 2018
    Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

H SinghDepartment of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA 19102, USA; Department of Anesthesiology, University of California, Los Angeles, CA 90095, USA. Electronic address: harpreet.singh@drexelmed.edu.
M LiDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA. Electronic address: mli@mednet.ucla.edu.
L HallDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA. Electronic address: halll@kenyon.edu.
S ChenDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA. Electronic address: scarlett.chen2009@gmail.com.
S SukurDepartment of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA 19102, USA. Electronic address: ss3773@drexel.edu.
R LuDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA. Electronic address: ronglu@ucla.edu.
A CaputoDepartment of Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA. Electronic address: annacaputo83@hotmail.it.
A L MeredithDepartment of Physiology, University of Maryland, Baltimore, MD 21201, USA. Electronic address: ameredith@som.umaryland.edu.
E StefaniDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA; Department of Physiology, University of California, Los Angeles, CA 90095, USA; Brain Research Institute, University of California, Los Angeles, CA 90095, USA. Electronic address: estefani@ucla.edu.
L ToroDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA; Brain Research Institute, University of California, Los Angeles, CA 90095, USA; Cardiovascular Research Laboratory, University of California, Los Angeles, CA 90095, USA. Electronic address: ltoro@g.ucla.edu.
University of California, Los Angeles · USDrexel University · USUniversity of Maryland, Baltimore · US

Funding

Daily Regulation of Ionic CurrentsR01HL102758 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI LEDERER, WILLIAM JONATHAN · 2010 to 2024
$6.3M
Revealing Cardiovascular Stress Regulation beyond the Diffraction LimitR01HL088640 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI STEFANI, ENRICO · 2007 to 2011
$5.3M
BK(Ca) channel in heart mitochondriaR01HL107418 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI OLCESE, RICCARDO, STEFANI, ENRICO · 2012 to 2015
$2.5M
Novel interactions of Slo1 channel and Thromboxane A2 receptor in blood vesselsR01HL096740 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI STEFANI, ENRICO, TORO DE STEFANI, LIGIA G. · 2009 to 2010
$1.3M
NHLBI NIH HHS HL096740NHLBI NIH HHS HL107418NHLBI NIH HHS R01 HL088640NHLBI NIH HHS R01 HL096740NHLBI NIH HHS R01 HL102758NHLBI NIH HHS R01 HL107418
6 · The paper itself

Abstract

Large conductance voltage and calcium-activated potassium (MaxiK) channels are activated by membrane depolarization and elevated cytosolic Ca(2+). In the brain, they localize to neurons and astrocytes, where they play roles such as resetting the membrane potential during an action potential, neurotransmitter release, and neurovascular coupling. MaxiK channels are known to associate with several modulatory proteins and accessory subunits, and each of these interactions can have distinct physiological consequences. To uncover new players in MaxiK channel brain physiology, we applied a directed proteomic approach and obtained MaxiK channel pore-forming α subunit brain interactome using specific antibodies. Controls included immunoprecipitations with rabbit immunoglobulin G (IgG) and with anti-MaxiK antibodies in wild type and MaxiK channel knockout mice (Kcnma1(-/-)), respectively. We have found known and unreported interactive partners that localize to the plasma membrane, extracellular space, cytosol and intracellular organelles including mitochondria, nucleus, endoplasmic reticulum and Golgi apparatus. Localization of MaxiK channel to mitochondria was further confirmed using purified brain mitochondria colabeled with MitoTracker. Independent proof of MaxiK channel interaction with previously unidentified partners is given for GABA transporter 3 (GAT3) and heat shock protein 60 (HSP60). In human embryonic kidney 293 cells containing SV40 T-antigen (HEK293T) cells, both GAT3 and HSP60 coimmunoprecipitated and colocalized with MaxiK channel; colabeling was observed mainly at the cell periphery with GAT3 and intracellularly with HSP60 with protein proximity indices of ∼ 0.6 and ∼ 0.4, respectively. In rat primary hippocampal neurons, colocalization index was identical for GAT3 (∼ 0.6) and slightly higher for HSP60 (∼ 0.5) association with MaxiK channel. The results of this study provide a complete interactome of MaxiK channel the mouse brain, further establish the localization of MaxiK channel in the mouse brain mitochondria and demonstrate the interaction of MaxiK channel with GAT3 and HSP60 in neurons. The interaction of MaxiK channel with GAT3 opens the possibility of a role of MaxiK channel in GABA homeostasis and signaling.

Indexed as

AnimalsAnimals, NewbornBrainCells, CulturedChaperonin 60Cyclooxygenase 2GABA Plasma Membrane Transport ProteinsGene Expression RegulationHEK293 CellsHippocampusHumansLarge-Conductance Calcium-Activated Potassium ChannelsMaleMiceMice, TransgenicMitochondriaChaperonin 60Cyclooxygenase 2GABA Plasma Membrane Transport ProteinsLarge-Conductance Calcium-Activated Potassium ChannelsPtgs2 protein, mouseBK(Ca)GABA transporterHSP60MaxiK interactomemitochondriaproteomics

Identifiers

PMID26772433
PMCPMC4737998
OpenAlexW2231434046

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.