Evidence map›Paper›PMID 26772160›Full record

ArticleAnalytical biochemistry2016

Design and development of high bioluminescent resonance energy transfer efficiency hybrid-imaging constructs.

Manoj Kumar, Letícia Kovalski, David Broyles, Eric A Hunt, Pirouz Daftarian, Emre Dikici, Sylvia Daunert, Sapna K Deo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Manoj KumarDepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA; Indian Institute of Technology (BHU), Department of Chemical Engineering and School of Biomedical Engineering, Varanasi, UP 221005, India.
Letícia KovalskiDepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
David BroylesDepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Eric A HuntDepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Pirouz DaftarianDepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Emre DikiciDepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Sylvia DaunertDepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Sapna K DeoDepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA. Electronic address: sdeo@med.miami.edu.

Funding

PHOTOPROTEINS AS LABELS IN BIOLUMINESCENCE ASSAYSR01GM047915 · NIGMS · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI DAUNERT, SYLVIA · 2000 to 2013
$3.5M
Sensitive Detection of Viral Persistency Using Bioluminescent Stem-Loop ProbesR01GM114321 · NIGMS · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI DEO, SAPNA K · 2015 to 2019
$1.5M
NIGMS NIH HHS R01 GM047915NIGMS NIH HHS R01 GM114321NIGMS NIH HHS R01GM114321
6 · The paper itself

Abstract

Here we describe the design and construction of an imaging construct with high bioluminescent resonance energy transfer (BRET) efficiency that is composed of multiple quantum dots (QDs; λem = 655 nm) self-assembled onto a bioluminescent protein, Renilla luciferase (Rluc). This is facilitated by the streptavidin-biotin interaction, allowing the facile formation of a hybrid-imaging construct (HIC) comprising up to six QDs (acceptor) grafted onto a light-emitting Rluc (donor) core. The resulting assembly of multiple acceptors surrounding a donor permits this construct to exhibit high resonance energy transfer efficiency (∼64.8%). The HIC was characterized using fluorescence excitation anisotropy measurements and high-resolution transmission electron microscopy. To demonstrate the application of our construct, a generation-5 (G5) polyamidoamine dendrimer (PAMAM) nanocarrier was loaded with our HIC for in vitro and in vivo imaging. We envision that this design of multiple acceptors and bioluminescent donor will lead to the development of new BRET-based systems useful in sensing, imaging, and other bioanalytical applications.

Indexed as

Drug DesignEnergy TransferLuminescent MeasurementsQuantum DotsDendrimersLuciferases, RenillaNanostructuresDendrimersLuciferases, RenillaPAMAM StarburstBioluminescenceBioluminescence resonance energy transferImaging constructQuantum dotsRenilla luciferase

Identifiers

PMID26772160
PMCPMC4829111

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.