ArticlePLoS neglected tropical diseases2016
Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 2 of them syntheses that pooled it.
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Who cites it
41 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Phlebotomine sand flies (Diptera: Psychodidae) of the Maghreb region: A systematic review of distribution, morphology, and role in the transmission of the pathogens.PLoS neglected tropical diseases · 2022Pooled it
- MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review.Parasites & vectors · 2019Pooled it
- Optimization of tick identification via MALDI-TOF MS for implementation in routine diagnostics.Parasitology research · 2026Article
- Ectoparasite survey of schoolchildren in the Republic of Guinea.PLOS global public health · 2026Article
- Article
- A Century of Epidemiological Advances in Cutaneous and Visceral Leishmaniasis in Algeria.Journal of parasitology research · 2025Review
- Efficiency of MALDI-TOF MS at identifying and discriminating immature stages of cimex lectularius and cimex hemipterus bed bugs.Scientific reports · 2024Article
- Article
- Potential of MALDI-TOF MS biotyping to detect deltamethrin resistance in the dengue vector Aedes aegypti.PloS one · 2024Article
- Species identification of phlebotomine sandflies using deep learning and wing interferential pattern (WIP).Scientific reports · 2023Article
- Evolution of MALDI-TOF MS Profiles from Lice and Fleas Preserved in Alcohol over Time.Insects · 2023Article
- Identification of NeotropicalTropical medicine and infectious disease · 2023Article
- Applications of MALDI-TOF Mass Spectrometry to the Identification of Parasites and Arthropod Vectors of Human Diseases.Microorganisms · 2022Review
- MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria.Microorganisms · 2022Article
- MALDI-TOF MS Limits for the Identification of Mediterranean Sandflies of the SubgenusMicroorganisms · 2022Article
- Article
- Enhanced procedures for mosquito identification by MALDI-TOF MS.Parasites & vectors · 2022Article
- MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.PLoS neglected tropical diseases · 2021Article
- Using MALDI-TOF mass spectrometry to identify ticks collected on domestic and wild animals from the Democratic Republic of the Congo.Experimental & applied acarology · 2021Article
- New assessment of Anopheles vector species identification using MALDI-TOF MS.Malaria journal · 2021Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhlebotomine sand flies are known to transmit Leishmania parasites, bacteria and viruses that affect humans and animals in many countries worldwide. Precise sand fly identification is essential to prevent phlebotomine-borne diseases. Over the past two decades, progress in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an accurate tool for arthropod identification. The objective of the present study was to investigate the usefulness of MALDI-TOF MS as a tool for identifying field-caught phlebotomine. METHODOLOGY/PRINCIPAL
findingsSand flies were captured in four sites in north Algeria. A subset was morphologically and genetically identified. Six species were found in these areas and a total of 28 stored frozen specimens were used for the creation of the reference spectrum database. The relevance of this original method for sand fly identification was validated by two successive blind tests including the morphological identification of 80 new specimens which were stored at -80°C, and 292 unknown specimens, including engorged specimens, which were preserved under different conditions. Intra-species reproducibility and inter-species specificity of the protein profiles were obtained, allowing us to distinguish specimens at the gender level. Querying of the sand fly database using the MS spectra from the blind test groups revealed concordant results between morphological and MALDI-TOF MS identification. However, MS identification results were less efficient for specimens which were engorged or stored in alcohol. Identification of 362 phlebotomine sand flies, captured at four Algerian sites, by MALDI-TOF MS, revealed that the subgenus Larroussius was predominant at all the study sites, except for in M'sila where P. (Phlebotomus) papatasi was the only sand fly species detected.
conclusionThe present study highlights the application of MALDI-TOF MS for monitoring sand fly fauna captured in the field. The low cost, reliability and rapidity of MALDI-TOF MS analyses opens up new ways in the management of phlebotomine sand fly-borne diseases.
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