Evidence map›Paper›PMID 26771487›Full record

ArticleCell2016

Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks.

Peter Bieling, Tai-De Li, Julian Weichsel, Ryan McGorty, Pamela Jreij, Bo Huang, Daniel A Fletcher, R Dyche Mullins

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 155 papers.

0numbers the graph read from it
0cells of the map it votes in
155citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

155 citing papers in PubMed.

  1. Review
  2. Mechanical regulation of cell memory.Nature structural & molecular biology · 2026
    Review
  3. Article
  4. Article
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  11. Biophysical Considerations in Cell Fusion.Advances in experimental medicine and biology · 2026
    Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Myosin 2 - A general contractor for the cytoskeleton.Current opinion in cell biology · 2025
    Review
  17. Review
  18. Physical confinement and phagocytic uptake induce directional migration.bioRxiv : the preprint server for biology · 2025
    Article
  19. Review
  20. Article

95 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Peter BielingDepartment of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, Genentech Hall, 600 16(th) Street, San Francisco, CA 94158, USA; Department of Bioengineering and Biophysics Program, University of California, Berkeley, 648 Stanley Hall MC 1762, Berkeley, CA 94720, USA.
Tai-De LiDepartment of Bioengineering and Biophysics Program, University of California, Berkeley, 648 Stanley Hall MC 1762, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, 648 Stanley Hall MC 1762, Berkeley, CA 94720, USA; Advance Science Research Center, City University of New York, 85 St. Nicholas Terrace, New York, NY 10031, USA.
Julian WeichselDepartment of Chemistry, University of California, Berkeley, 207 Gilman Hall, Berkeley, CA 94720, USA.
Ryan McGortyDepartment of Biochemistry and Biophysics, University of California, San Francisco, Genentech Hall, 600 16(th) Street, San Francisco, CA 94158, USA.
Pamela JreijDepartment of Bioengineering and Biophysics Program, University of California, Berkeley, 648 Stanley Hall MC 1762, Berkeley, CA 94720, USA.
Bo HuangDepartment of Biochemistry and Biophysics, University of California, San Francisco, Genentech Hall, 600 16(th) Street, San Francisco, CA 94158, USA.
Daniel A FletcherDepartment of Bioengineering and Biophysics Program, University of California, Berkeley, 648 Stanley Hall MC 1762, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, 648 Stanley Hall MC 1762, Berkeley, CA 94720, USA. Electronic address: fletch@berkeley.edu.
R Dyche MullinsDepartment of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, Genentech Hall, 600 16(th) Street, San Francisco, CA 94158, USA. Electronic address: dyche.mullins@ucsf.edu.

Funding

Assembly and function of cytoskeletal systems in eukaryotic and prokaryoticcellsR35GM118119 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MULLINS, R DYCHE · 2016 to 2025
$4.8M
MECHANISMS OF ARP2/3 ACTIVATION AND FUNCTIONR01GM061010 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MULLINS, R DYCHE · 2000 to 2015
$3.8M
Mechanical Regulation of Actin NetworksR01GM074751 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI FLETCHER, DANIEL A · 2008 to 2015
$2.4M
Solving macromolecular complex architecture in situ by super-resolution microscopDP2OD008479 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, BO · 2011 to 2011
$2.3M
Howard Hughes Medical InstituteNIGMS NIH HHS R01 GM061010NIGMS NIH HHS R01 GM074751NIGMS NIH HHS R35 GM118119NIH HHS DP2 OD008479
6 · The paper itself

Abstract

Branched actin networks--created by the Arp2/3 complex, capping protein, and a nucleation promoting factor--generate and transmit forces required for many cellular processes, but their response to force is poorly understood. To address this, we assembled branched actin networks in vitro from purified components and used simultaneous fluorescence and atomic force microscopy to quantify their molecular composition and material properties under various forces. Remarkably, mechanical loading of these self-assembling materials increases their density, power, and efficiency. Microscopically, increased density reflects increased filament number and altered geometry but no change in average length. Macroscopically, increased density enhances network stiffness and resistance to mechanical failure beyond those of isotropic actin networks. These effects endow branched actin networks with memory of their mechanical history that shapes their material properties and motor activity. This work reveals intrinsic force feedback mechanisms by which mechanical resistance makes self-assembling actin networks stiffer, stronger, and more powerful.

Indexed as

Actin-Related Protein 2-3 ComplexActinsBiomechanical PhenomenaHumansMicroscopy, Atomic ForceMicroscopy, FluorescenceThermodynamicsWiskott-Aldrich Syndrome Protein FamilyActin-Related Protein 2-3 ComplexActinsWASF1 protein, humanWiskott-Aldrich Syndrome Protein Family

Identifiers

PMID26771487
PMCPMC5033619

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.