Evidence map›Paper›PMID 26771074›Full record

ArticleScientific reports2016

Novel Methods of Determining Urinary Calculi Composition: Petrographic Thin Sectioning of Calculi and Nanoscale Flow Cytometry Urinalysis.

Carson T Gavin, Sohrab N Ali, Thomas Tailly, Daniel Olvera-Posada, Husain Alenezi, Nicholas E Power, Jinqiang Hou, Andre H St Amant, Leonard G Luyt, Stephen Wood and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Disease models & mechanisms · 2018
    Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Carson T GavinDepartment of Surgery, Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.
Sohrab N AliDepartment of Surgery, Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.
Thomas TaillyDepartment of Surgery, Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.
Daniel Olvera-PosadaDepartment of Surgery, Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.
Husain AleneziDepartment of Surgery, Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.
Nicholas E PowerDepartment of Surgery, Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.
Jinqiang HouDepartment of Chemistry, Western University, London, Ontario, Canada.
Andre H St AmantDepartment of Chemistry, Western University, London, Ontario, Canada.
Leonard G LuytDepartment of Chemistry, Western University, London, Ontario, Canada.
Stephen WoodDepartment of Earth Sciences, Western University, London, Ontario, Canada.
Charles WuDepartment of Earth Sciences, Western University, London, Ontario, Canada.
Hassan RazviDepartment of Surgery, Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.
Hon S LeongDepartment of Surgery, Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.
Western University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate determination of urinary stone composition has significant bearing on understanding pathophysiology, choosing treatment modalities and preventing recurrence. A need exists for improved methods to determine stone composition. Urine of 31 patients with known renal calculi was examined with nanoscale flow cytometry and the calculi collected during surgery subsequently underwent petrographic thin sectioning with polarized and fluorescent microscopy. Fluorescently labeled bisphosphonate probes (Alendronate-fluorescein/Alendronate-Cy5) were developed for nanoscale flow cytometry to enumerate nanocrystals that bound the fluorescent probes. Petrographic sections of stones were also imaged by fluorescent and polarized light microscopy with composition analysis correlated to alendronate +ve nanocrystal counts in corresponding urine samples. Urine samples from patients with Ca(2+) and Mg(2+) based calculi exhibited the highest alendronate +ve nanocrystal counts, ranging from 100-1000 nm in diameter. This novel urine based assay was in agreement with composition determined by petrographic thin sections with Alendronate probes. In some cases, high alendronate +ve nanocrystal counts indicated a Ca(2+) or Mg(2+) composition, as confirmed by petrographic analysis, overturning initial spectrophotometric diagnosis of stone composition. The combination of nanoscale flow cytometry and petrographic thin sections offer an alternative means for determining stone composition. Nanoscale flow cytometry of alendronate +ve nanocrystals alone may provide a high-throughput means of evaluating stone burden.

Indexed as

Flow CytometryFluorescent DyesHumansKidney CalculiMicroscopy, FluorescenceMicrotomyUrinalysisUrinary CalculiFluorescent Dyes

Identifiers

PMID26771074
PMCPMC4725893
OpenAlexW2428429306

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.