ArticleOxidative medicine and cellular longevity2016
Sperm Oxidative Stress Is Detrimental to Embryo Development: A Dose-Dependent Study Model and a New and More Sensitive Oxidative Status Evaluation.
Article in Oxidative medicine and cellular longevity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 118 citations in OpenAlex.
- Trial
- Heat Stress and the Bull Sperm Transcriptome: Molecular Mechanisms, Fertility, and Epigenetic Inheritance.Animals : an open access journal from MDPI · 2026Review
- Effect of low-temperature stress on sperm DNA methylation and oxidative damage during cryopreservation of Tibetan pig semen.Veterinary world · 2026Article
- Effects of fertilization media on in vitro embryonic development in red-rumped agouti () using a heterologous system with guinea pig oocytes.JBRA assisted reproduction · 2026Article
- From sperm to pregnancy: insights into paternal regulation of fertility divergence in cattle.The Journal of reproduction and development · 2026Review
- How Early-Life Programming During Embryogenesis Imprints Cellular Memory.International journal of molecular sciences · 2025Review
- Glucose and pyruvate differentially modulate metabolic and redox dynamics during capacitation to enable fertilization competence in mouse sperm.Reproductive biology and endocrinology : RB&E · 2025Article
- Parental dietary patterns and assisted reproductive technology outcomes including embryo morphokinetics: rotterdam periconception cohort.Journal of assisted reproduction and genetics · 2025Article
- Age-related decline in behavior and reproductive health in male mice.Scientific reports · 2025Article
- Oxidative Damage Under Microgravity Conditions: Response Mechanisms, Monitoring Methods and Countermeasures on Somatic and Germ Cells.International journal of molecular sciences · 2025Review
- Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen-Thawed Semen from Rams.Animals : an open access journal from MDPI · 2025Article
- Impact of paternal high energy diets on semen quality and embryo development in cattle.Reproduction & fertility · 2025Review
- Selecting sires to improve reproductive success: key traits for enhanced fertility and embryo development.Animal reproduction · 2025Article
- Pyrroroquinoline Quinone (PQQ) Improves the Quality of Holstein Bull Semen during Cryopreservation.Animals : an open access journal from MDPI · 2024Article
- Supplementation with MitoTEMPO before cryopreservation improves sperm quality and fertility potential of Piedmontese beef bull semen.Frontiers in veterinary science · 2024Article
- The characterization of CellROX™ probes could be a crucial factor in ram sperm quality assessment.Frontiers in veterinary science · 2024Article
- Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos.Animals : an open access journal from MDPI · 2023Article
- Review of optical reporters of radiation effectsJournal of biomedical optics · 2023Review
- Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm.Animals : an open access journal from MDPI · 2023Article
- CanFrontiers in veterinary science · 2023Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our study aimed to assess the impact of sperm oxidative stress on embryo development by means of a dose-dependent model. In experiment 1, straws from five bulls were subjected to incubation with increasing H2O2 doses (0, 12.5, 25, and 50 μM). Motility parameters were evaluated by Computed Assisted System Analysis (CASA). Experiment 2 was designed to study a high (50 μM) and low dose (12.5 μM) of H2O2 compared to a control (0 μM). Samples were incubated and further used for in vitro fertilization. Analyses of motility (CASA), oxidative status (CellROX green and 2'-7' dichlorofluorescein diacetate), mitochondrial potential (JC-1), chromatin integrity (AO), and sperm capacitation status (chlortetracycline) were performed. Embryos were evaluated based on fast cleavage (30 h.p.i.), cleavage (D = 3), development (D = 5), and blastocyst rates (D = 8). We observed a dose-dependent deleterious effect of H2O2 on motility and increase on the percentages of positive cells for CellROX green, capacitated sperm, and AO. A decrease on cleavage and blastocyst rates was observed as H2O2 increased. Also, we detected a blockage on embryo development. We concluded that sperm when exposed to oxidative environment presents impaired motility traits, prooxidative status, and premature capacitation; such alterations resulting in embryo development fail.
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