Evidence map›Paper›PMID 26770521›Full record

ArticleInternational journal of clinical and experimental medicine2015

Polyphylla saponin I has antiviral activity against influenza A virus.

Xiuying Pu, Jing Ren, Xiaolong Ma, Lu Liu, Shuang Yu, Xiaoyue Li, Haibing Li

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

  1. The biosynthesis of trillin 6'-Synthetic and systems biotechnology · 2025
    Article
  2. Review
  3. Article
  4. Review
  5. COVID-19: Eco-friendly hand hygiene for human and environmental safety.Journal of environmental chemical engineering · 2021
    Review
  6. Review
  7. Current strategies against COVID-19.Chinese medicine · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Xiuying PuCollege of Life Science and Engineering, Lanzhou University of TechnologyLanzhou 730050, P. R. China; The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Education Department of Gansu Provincial GovernmentLanzhou 730050, P. R. China.
Jing RenCollege of Life Science and Engineering, Lanzhou University of TechnologyLanzhou 730050, P. R. China; The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Education Department of Gansu Provincial GovernmentLanzhou 730050, P. R. China.
Xiaolong MaCollege of Life Science and Engineering, Lanzhou University of TechnologyLanzhou 730050, P. R. China; The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Education Department of Gansu Provincial GovernmentLanzhou 730050, P. R. China.
Lu LiuCollege of Life Science and Engineering, Lanzhou University of TechnologyLanzhou 730050, P. R. China; The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Education Department of Gansu Provincial GovernmentLanzhou 730050, P. R. China.
Shuang YuCollege of Life Science and Engineering, Lanzhou University of TechnologyLanzhou 730050, P. R. China; The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Education Department of Gansu Provincial GovernmentLanzhou 730050, P. R. China.
Xiaoyue LiCollege of Life Science and Engineering, Lanzhou University of TechnologyLanzhou 730050, P. R. China; The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Education Department of Gansu Provincial GovernmentLanzhou 730050, P. R. China.
Haibing LiCollege of Life Science and Engineering, Lanzhou University of TechnologyLanzhou 730050, P. R. China; The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Education Department of Gansu Provincial GovernmentLanzhou 730050, P. R. China.
Lanzhou University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveIn the present study, the antiviral effects of polyphylla saponin I isolated from Parispolyphylla on influenza A virus are investigated both in vitro and in vivo.

methodsColumn chromatography and reversed phase liquid chromatography separation technology were used to extract and purify polyphylla saponin I. The purity of polyphylla saponin I was assayed by high performance liquid chromatography. Methyl thiazolyl tetrazolium assay and analyses of cytopathic effects were performed to examine the antiviral activity of polyphylla saponin I upon MDCK cells infected with influenza A virus. Model mice were made by intranasal inoculation of influenza a virus. Mice infected with influenza A virus were orally administered polyphylla saponin I and oseltamivir twice a day for 5 days to study their antiviral efficacy in vivo.

resultsPolyphylla saponin I had no cytotoxicity on MDCK cells at the concentration of 50 μg/mL. Polyphylla saponin I (6.25, 12.5, 25 and 50 μg/mL) and oseltamivir (40 μg/mL) had remarkable inactivation effects on influenza A virus, prevention effects on influenza A virus adsorption on MDCK cells, and inhibitory effects on the reproduction of influenza A virus in MDCK cells. In addition, polyphylla saponin I (5 and 10 mg/kg), and oseltamivir (3 mg/kg) significantly reduced viral hemagglutination titer, improved the pathologic histology of lung tissues, and decreased the mortality of mice infected with influenza A virus. Polyphylla saponin I (5 and 10 mg/kg) prolonged the survival time of mice from 8.5±0.3 days to 13.2±0.5 days, with the prolonged life rates being 49.4% and 55.3%, respectively.

conclusionPolyphylla saponin I has antiviral activity on influenza A virus both in vitro and in vivo.

Indexed as

antiviral activityInfluenza A virusoseltamivirpolyphylla saponin I

Identifiers

PMID26770521
PMCPMC4694421
OpenAlexW2411410754

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.