ArticleInternational journal of clinical and experimental medicine2015
16S rRNA gene sequencing is a non-culture method of defining the specific bacterial etiology of ventilator-associated pneumonia.
Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- The Role of the Vaginal Microbiome in Preterm Premature Rupture of Membranes: A Comprehensive Review of Mechanisms and Clinical Implications.Health science reports · 2025Article
- Identification of bacterial pathogens in sudden unexpected death in infancy and childhood using 16S rRNA gene sequencing.Frontiers in microbiology · 2023Article
- Composition and diversity analysis of the lung microbiome in patients with suspected ventilator-associated pneumonia.Critical care (London, England) · 2022Article
- Clinical characteristics and drug susceptibility patterns of Corynebacterium species in bacteremic patients with hematological disorders.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Extensive microbiological respiratory tract specimen characterization in critically ill COVID-19 patients.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2021Observational
- Respiratory microbiome profiles differ by recent hospitalization and nursing home residence in patients on mechanical ventilation.Journal of translational medicine · 2020Article
- Letter to the Editor concerning "Bacteria: back pain, leg pain and Modic sign-a surgical multicentre comparative study" by Fritzell P, et al. (Eur Spine J [2019]; doi:10.1007/s00586-019-06164-1).European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2020Article
- Rapid analysis of bacterial composition in prosthetic joint infection by 16S rRNA metagenomic sequencing.Bone & joint research · 2019Article
- 16SPIP: a comprehensive analysis pipeline for rapid pathogen detection in clinical samples based on 16S metagenomic sequencing.BMC bioinformatics · 2017Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ventilator-associated pneumonia (VAP) is an acquired respiratory tract infection following tracheal intubation. The most common hospital-acquired infection among patients with acute respiratory failure, VAP is associated with a mortality rate of 20-30%. The standard bacterial culture method for identifying the etiology of VAP is not specific, timely, or accurate in identifying the bacterial pathogens. This study used 16S rRNA gene metagenomic sequencing to identify and quantify the pathogenic bacteria in lower respiratory tract and oropharyngeal samples of 55 VAP patients. Sequencing of the 16S rRNA gene has served as a valuable tool in bacterial identification, particularly when other biochemical, molecular, or phenotypic identification techniques fail. In this study, 16S rRNA gene sequencing was performed in parallel with the standard bacterial culture method to identify and quantify bacteria present in the collected patient samples. Sequence analysis showed the colonization of multidrug-resistant strains in VAP secretions. Further, this method identified Prevotella, Proteus, Aquabacter, and Sphingomonas bacterial genera that were not detected by the standard bacterial culture method. Seven categories of bacteria, Streptococcus, Neisseria, Corynebacterium, Acinetobacter, Staphylococcus, Pseudomonas and Klebsiella, were detectable by both 16S rRNA gene sequencing and standard bacterial culture methods. Further, 16S rRNA gene sequencing had a significantly higher sensitivity in detecting Streptococcus and Pseudomonas when compared to standard bacterial culture. Together, these data present 16S rRNA gene sequencing as a novel VAP diagnosis tool that will further enable pathogen-specific treatment of VAP.
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26770469PMC4694369W2412462941What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.