ArticleStem cells international2016
Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood.
Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Flavonoids and Alzheimer's disease: reviewing the evidence for neuroprotective potential.Molecular and cellular biochemistry · 2025Review
- Isolation, Expansion, and Characterization of Rat Hair Follicle Stem Cells and Their Secretome: Insights into Wound Healing Potential.Biomedicines · 2024Article
- Orthobiologic Products: Preservation Options for Orthopedic Research and Clinical Applications.Journal of clinical medicine · 2024Review
- Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities.RSC medicinal chemistry · 2024Review
- Optimization of Culture Medium for the Production of an Exopolysaccharide (p-CY02) with Cryoprotective Activity byJournal of microbiology and biotechnology · 2024Article
- Development of Serum-Free Media for Cryopreservation of Hydrogel Encapsulated Cell-Based Therapeutics.Cellular and molecular bioengineering · 2022Article
- Article
- On crystallization of water confined in liposomes and cryoprotective action of DMSO.RSC advances · 2022Article
- Effects of storage media, supplements and cryopreservation methods on quality of stem cells.World journal of stem cells · 2021Review
- The combination of trehalose and glycerol: an effective and non-toxic recipe for cryopreservation of human adipose-derived stem cells.Stem cell research & therapy · 2020Article
- Cryopreservation of Hematopoietic Stem Cells: Emerging Assays, Cryoprotectant Agents, and Technology to Improve Outcomes.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019Review
- Bioinspired Preservation of Natural Killer Cells for Cancer Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- To Protect and to Preserve: Novel Preservation Strategies for Extracellular Vesicles.Frontiers in pharmacology · 2018Review
- Effect of trehalose on cryopreservation of pure peripheral blood stem cells.Biomedical reports · 2017Article
- Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model.Stem cells international · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was divided into four equal parts and frozen in different kinds of cryoprotectant as follows: group A, 10% ethylene glycol and 2.0% dimethyl sulfoxide (DMSO) (v/v); group B, 10% DMSO and 2.0% dextran-40; group C, 2.5% DMSO (v/v) + 30 mmol/L trehalose; and group D, without CPA. Results. CD34(+), cell viability, colony forming units (CFUs), and cell apoptosis of pre- and postcryopreservation using three cryoprotectants were analyzed. After thawing, significant differences in CD34(+) count, CFUs, cell apoptosis, and cell viability were observed among the four groups (P < 0.05). Conclusion. The low concentration of DMSO with the addition of trehalose might improve the cryopreservation outcome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.