ArticleChembiochem : a European journal of chemical biology2016
The Phormidolide Biosynthetic Gene Cluster: A trans-AT PKS Pathway Encoding a Toxic Macrocyclic Polyketide.
Article in Chembiochem : a European journal of chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Targeted Discovery of β‑Branched Conjugated Polyketides from Bacteria Based on Genomic and Metabolomic Hallmarks.JACS Au · 2026Article
- β-Branching in the biosynthesis of bongkrekic acid: a complex affair.RSC advances · 2025Article
- Leptochelins A-C, Cytotoxic Metallophores Produced by Geographically DispersedJournal of the American Chemical Society · 2024Article
- Evolution-guided engineering ofScience (New York, N.Y.) · 2024Article
- Enzymatic Halogenation of Terminal Alkynes.Journal of the American Chemical Society · 2023Article
- Evolution of combinatorial diversity in trans-acyltransferase polyketide synthase assembly lines across bacteria.Nature communications · 2021Article
- Bioinformatic and Mechanistic Analysis of the Palmerolide PKS-NRPS Biosynthetic Pathway From the Microbiome of an Antarctic Ascidian.Frontiers in chemistry · 2021Article
- The Chemistry, Biochemistry and Pharmacology of Marine Natural Products fromMarine drugs · 2020Review
- Synergism of anisotropic and computational NMR methods reveals the likely configuration of phormidolide A.Chemical communications (Cambridge, England) · 2020Article
- Automated structure prediction of trans-acyltransferase polyketide synthase products.Nature chemical biology · 2019Article
- Structural and Functional Studies of a gem-Dimethylating Methyltransferase from a trans-Acyltransferase Assembly Line.ACS chemical biology · 2018Article
- Trichophycins B-F, Chlorovinylidene-Containing Polyketides Isolated from a Cyanobacterial Bloom.The Journal of organic chemistry · 2018Article
- A Polyketide Synthase Component for Oxygen Insertion into Polyketide Backbones.Angewandte Chemie (International ed. in English) · 2018Article
- Structural and Functional Studies of a Pyran Synthase Domain from a trans-Acyltransferase Assembly Line.ACS chemical biology · 2018Article
- The Swinholide Biosynthesis Gene Cluster from a Terrestrial Cyanobacterium, Nostoc sp. Strain UHCC 0450.Applied and environmental microbiology · 2018Article
- Polyketide stereocontrol: a study in chemical biology.Beilstein journal of organic chemistry · 2017Review
- Integrating mass spectrometry and genomics for cyanobacterial metabolite discovery.Journal of industrial microbiology & biotechnology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
Phormidolide is a polyketide produced by a cultured filamentous marine cyanobacterium and incorporates a 16-membered macrolactone. Its complex structure is recognizably derived from a polyketide synthase pathway, but possesses unique and intriguing structural features that prompted interest in investigating its biosynthetic origin. Stable isotope incorporation experiments confirmed the polyketide nature of this compound. We further characterized the phormidolide gene cluster (phm) through genome sequencing followed by bioinformatic analysis. Two discrete trans-type acyltransferase (trans-AT) ORFs along with KS-AT adaptor regions (ATd) within the polyketide synthase (PKS) megasynthases, suggest that the phormidolide gene cluster is a trans-AT PKS. Insights gained from analysis of the mode of acetate incorporation and ensuing keto reduction prompted our reevaluation of the stereochemistry of phormidolide hydroxy groups located along the linear polyketide chain.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.