ArticleScientific reports2016
Identification of a novel family of carbohydrate-binding modules with broad ligand specificity.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Gatekeeping Dietary Fiber: The Role of Carbohydrate-Binding Modules in the Human Gut.Journal of microbiology and biotechnology · 2026Review
- Identification of endoglucanase-encoding genes from goat rumen bacterial metagenomes using homology- and motif-based approaches.Archives of microbiology · 2025Article
- Microbial carbohydrate active enzyme (CAZyme) genes and diversity from Menagesha Suba natural forest soils of Ethiopia as revealed by shotgun metagenomic sequencing.BMC microbiology · 2024Article
- Harnessing cellulose-binding protein domains for the development of functionalized cellulose materials.Bioresources and bioprocessing · 2024Review
- CORK1, A LRR-Malectin Receptor Kinase, Is Required for Cellooligomer-Induced Responses inCells · 2022Article
- Unique properties of a Dictyostelium discoideum carbohydrate-binding module expand our understanding of CBM-ligand interactions.The Journal of biological chemistry · 2022Article
- Emerging Roles of β-Glucanases in Plant Development and Adaptative Responses.Plants (Basel, Switzerland) · 2022Review
- Characterization of an Unknown Region Linked to the Glycoside Hydrolase Family 17 β-1,3-Glucanase ofMarine drugs · 2022Article
- From Cancer Therapy to Winemaking: The Molecular Structure and Applications of β-Glucans and β-1, 3-Glucanases.International journal of molecular sciences · 2022Review
- Comparative Evaluation of Adsorption of Major Enzymes in a Cellulase Cocktail Obtained fromPolymers · 2022Article
- Malectin/Malectin-like domain-containing proteins: A repertoire of cell surface molecules with broad functional potential.Cell surface (Amsterdam, Netherlands) · 2021Review
- A novel amylolytic/xylanolytic/cellulolytic multienzyme complex from Clostridium manihotivorum that hydrolyzes polysaccharides in cassava pulp.Applied microbiology and biotechnology · 2021Article
- Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.Journal of molecular modeling · 2019Article
- Origin, evolution, and divergence of plant class C GH9 endoglucanases.BMC evolutionary biology · 2018Article
- Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most enzymes that act on carbohydrates include non-catalytic carbohydrate-binding modules (CBMs) that recognize and target carbohydrates. CBMs bring their appended catalytic modules into close proximity with the target substrate and increase the hydrolytic rate of enzymes acting on insoluble substrates. We previously identified a novel CBM (CBMC5614-1) at the C-terminus of endoglucanase C5614-1 from an uncultured microorganism present in buffalo rumen. In the present study, that the functional region of CBMC5614-1 involved in ligand binding was localized to 134 amino acids. Two representative homologs of CBMC5614-1, sharing the same ligand binding profile, targeted a range of β-linked polysaccharides that adopt very different conformations. Targeted substrates included soluble and insoluble cellulose, β-1,3/1,4-mixed linked glucans, xylan, and mannan. Mutagenesis revealed that three conserved aromatic residues (Trp-380, Tyr-411, and Trp-423) play an important role in ligand recognition and targeting. These results suggest that CBMC5614-1 and its homologs form a novel CBM family (CBM72) with a broad ligand-binding specificity. CBM72 members can provide new insight into CBM-ligand interactions and may have potential in protein engineering and biocatalysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.