ArticlePloS one2016
Detection of MET Gene Copy Number in Cancer Samples Using the Droplet Digital PCR Method.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Exploring copy number alterations (CNAs) to gain new insight into SCLC heterogeneity.Molecular biology reports · 2026Review
- Droplet Digital PCR Improves Detection ofInternational journal of molecular sciences · 2025Article
- Research on quantitative detection technology of raccoon-derived ingredient adulteration in sausage products.Food science & nutrition · 2024Article
- Plasma ddPCR for the detection ofTherapeutic advances in medical oncology · 2024Article
- Chinese expert consensus on clinical practice of MET detection in non-small cell lung cancer.Therapeutic advances in medical oncology · 2024Review
- Development of a droplet digital PCR method for detection of porcine circovirus 4.BMC veterinary research · 2023Article
- Detection ofCancer research communications · 2023Article
- A novel method to assess copy number variation in melanoma: Droplet digital PCR for precise quantitation of the RREB1 gene in formalin-fixed, paraffin-embedded melanocytic neoplasms, a proof-of-concept study.Journal of cutaneous pathology · 2023Article
- MET Amplification as a Resistance Driver to TKI Therapies in Lung Cancer: Clinical Challenges and Opportunities.Cancers · 2023Review
- Validation of droplet digital PCR for cytokeratin 19 mRNA detection in canine peripheral blood and mammary gland.Scientific reports · 2022Article
- Development and use of a droplet digital PCR (ddPCR) assay to achieve sensitive and fast atypical porcine pestivirus detection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Copy Number Alterations as Novel Biomarkers and Therapeutic Targets in Colorectal Cancer.Cancers · 2022Review
- Multiplex Droplet Digital PCR Assay for Detection ofCancers · 2022Article
- MET alterations in advanced pulmonary sarcomatoid carcinoma.Frontiers in oncology · 2022Review
- The NHanceBiomedicines · 2021Article
- Has programmed cell death ligand-1Translational lung cancer research · 2021Review
- ddPCR Analysis Reveals BRAF V600E Mutations Are Infrequent in Isolated Pituitary Langerhans Cell Histiocytosis Patients.Journal of neuropathology and experimental neurology · 2020Article
- MET-dependent solid tumours - molecular diagnosis and targeted therapy.Nature reviews. Clinical oncology · 2020Review
- Application of droplet digital PCR for the detection of vector copy number in clinical CAR/TCR T cell products.Journal of translational medicine · 2020Article
- Detection of MET Alterations Using Cell Free DNA and Circulating Tumor Cells from Cancer Patients.Cells · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe analysis of MET gene copy number (CN) has been considered to be a potential biomarker to predict the response to MET-targeted therapies in various cancers. However, the current standard methods to determine MET CN are SNP 6.0 in the genomic DNA of cancer cell lines and fluorescence in situ hybridization (FISH) in tumor models, respectively, which are costly and require advanced technical skills and result in relatively subjective judgments. Therefore, we employed a novel method, droplet digital PCR (ddPCR), to determine the MET gene copy number with high accuracy and precision.
methodsThe genomic DNA of cancer cell lines or tumor models were tested and compared with the MET gene CN and MET/CEN-7 ratio determined by SNP 6.0 and FISH, respectively.
resultsIn cell lines, the linear association of the MET CN detected by ddPCR and SNP 6.0 is strong (Pearson correlation = 0.867). In tumor models, the MET CN detected by ddPCR was significantly different between the MET gene amplification and non-amplification groups according to FISH (mean: 15.4 vs 2.1; P = 0.044). Given that MET gene amplification is defined as MET CN >5.5 by ddPCR, the concordance rate between ddPCR and FISH was 98.0%, and Cohen's kappa coefficient was 0.760 (95% CI, 0.498-1.000; P <0.001).
conclusionsThe results demonstrated that the ddPCR method has the potential to quantify the MET gene copy number with high precision and accuracy as compared with the results from SNP 6.0 and FISH in cancer cell lines and tumor samples, respectively.
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