ArticlePloS one2016
Thermal Destabilization of Collagen Matrix Hierarchical Structure by Freeze/Thaw.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Effects of Preservation on the Compressive Properties and Microstructure of the Knee Meniscus.Annals of biomedical engineering · 2026Article
- Biomechanical comparison of high hydrostatic pressure versus liquid nitrogen treatment for devitalized bone grafts: a fresh porcine model study.BMC musculoskeletal disorders · 2026Article
- Structure-functionality relationship of collagen-fibrin interpenetrating hydrogels for engineered tumor-stroma models.Acta biomaterialia · 2026Article
- Microwave-assisted immunostaining for rapid labeling of matrix-embedded multicellular structures.APL bioengineering · 2025Article
- Greener Decellularization of Porcine Auricular Cartilage Using Supercritical Technology and Different Pretreatments for Application in Tissue Engineering.ACS biomaterials science & engineering · 2025Article
- Impact of storage techniques on ovine temporomandibular joint discs composition and physicochemical properties.Frontiers in bioengineering and biotechnology · 2025Article
- Advances in the application of hydrogel-based scaffolds for tendon repair.Genes & diseases · 2024Review
- The biomechanical and biological effect of supercooling on cortical bone allograft.Journal of veterinary science · 2023Article
- Tailoring the multiscale mechanics of tunable decellularized extracellular matrix (dECM) for wound healing through immunomodulation.Bioactive materials · 2023Article
- Pharmaceutical Development of Nanostructured Vesicular Hydrogel Formulations of Rifampicin for Wound Healing.International journal of molecular sciences · 2022Article
- Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues.Nature communications · 2022Article
- The Texture Change of Chinese Traditional Pig Trotter with Soy Sauce during Stewing Processing: Based on a Thermal Degradation Model of Collagen Fibers.Foods (Basel, Switzerland) · 2022Article
- Composite Inks for Extrusion Printing of Biological and Biomedical Constructs.ACS biomaterials science & engineering · 2021Review
- Cartilage Assessment Requires a Surface Characterization Protocol: Roughness, Friction, and Function.Tissue engineering. Part C, Methods · 2021Article
- Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3DInternational journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group · 2021Article
- Self-assembly study of type I collagen extracted from male Wistar Hannover rat tail tendons.Biomaterials research · 2020Article
- Modification of Collagen/Gelatin/Hydroxyethyl Cellulose-Based Materials by Addition of Herbal Extract-Loaded Microspheres Made from Gellan Gum and Xanthan Gum.Materials (Basel, Switzerland) · 2020Article
- Thermally-controlled extrusion-based bioprinting of collagen.Journal of materials science. Materials in medicine · 2019Article
- Label-free assessment of carotid artery biochemical composition using fiber-based fluorescence lifetime imaging.Biomedical optics express · 2018Article
- Freezing does not alter multiscale tendon mechanics and damage mechanisms in tension.Annals of the New York Academy of Sciences · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
This study aims to characterize and understand the effects of freezing on collagen structures and functionality. Specifically, thermodynamic destabilization of collagen at molecular- and fibril-levels by combination of low temperatures and freezing were experimentally characterized using modulated differential scanning calorimetry. In order to delineate the effects of sub-zero temperature and water-ice phase change, we hypothesized that the extent of destabilization can be determined based on post-thaw heat induced thermal denaturation of collagen. It is found that thermal denaturation temperature of collagen in hydrogel decreases by 1.4-1.6°C after freeze/thaw while no such decrease is observed in the case of molecular solution. The destabilization is predominantly due to ice formation. Exposure to low temperatures in the absence of ice has only minimal effect. Calorimetry measurements combined with morphological examination of collagen matrices by scanning electron microscopy suggest that freezing results in destabilization of collagen fibrils due to expansion of intrafibrillar space by ice formation. This fibril-level damage can be alleviated by use of cryoprotectant DMSO at concentrations as low as 0.5 M. A theoretical model explaining the change in collagen post-thaw thermal stability by freezing-induced fibril expansion is also proposed.
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Registered trials
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