ArticlePloS one2016
Spatially Resolved Quantification of Chromatin Condensation through Differential Local Rheology in Cell Nuclei Fluorescence Lifetime Imaging.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Chromatin remodelling: a driving force in reverse mechanotransduction.RNA biology · 2026Review
- Material-Induced Nuclear Deformation Controls Chromatin Architecture in Adipose Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels.npj biological physics and mechanics · 2026Article
- Chromatin heterogeneity modulates nuclear condensate dynamics and phase behavior.Nature communications · 2025Article
- Mechanobiology of the nucleus during the G2-M transition.Nucleus (Austin, Tex.) · 2024Review
- Image-based discrimination of the early stages of mesenchymal stem cell differentiation.Molecular biology of the cell · 2024Article
- Fluorescence saturation imaging microscopy: molecular fingerprinting with a standard confocal microscope.Biomedical optics express · 2024Article
- Distinct cytoskeletal regulators of mechanical memory in cardiac fibroblasts and cardiomyocytes.Basic research in cardiology · 2024Article
- Chromatin condensation regulates endothelial cell adaptation to shear stress.Molecular biology of the cell · 2022Article
- Mechanical Forces in Nuclear Organization.Cold Spring Harbor perspectives in biology · 2022Review
- Structure of mitotic chromosomes.Molecular cell · 2021Article
- Fluorescence lifetime imaging for studying DNA compaction and gene activities.Light, science & applications · 2021Article
- Orchestration of Force Generation and Nuclear Collapse in Apoptotic Cells.International journal of molecular sciences · 2021Review
- Bisphenol A, Bisphenol F, and Bisphenol S: The Bad and the Ugly. Where Is the Good?Life (Basel, Switzerland) · 2021Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Cannabinoid Combination Induces Cytoplasmic Vacuolation in MCF-7 Breast Cancer Cells.Molecules (Basel, Switzerland) · 2020Article
- Article
- Multiple particle tracking analysis in isolated nuclei reveals the mechanical phenotype of leukemia cells.Scientific reports · 2020Article
- SMURF2 prevents detrimental changes to chromatin, protecting human dermal fibroblasts from chromosomal instability and tumorigenesis.Oncogene · 2020Article
- Effect of Nuclear Stiffness on Cell Mechanics and Migration of Human Breast Cancer Cells.Frontiers in cell and developmental biology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The linear sequence of DNA encodes access to the complete set of proteins that carry out cellular functions. Yet, much of the functionality appropriate for each cell is nested within layers of dynamic regulation and organization, including a hierarchy of chromatin structural states and spatial arrangement within the nucleus. There remain limitations in our understanding of gene expression within the context of nuclear organization from an inability to characterize hierarchical chromatin organization in situ. Here we demonstrate the use of fluorescence lifetime imaging microscopy (FLIM) to quantify and spatially resolve chromatin condensation state using cell-permeable, DNA-binding dyes (Hoechst 33342 and PicoGreen). Through in vitro and in situ experiments we demonstrate the sensitivity of fluorescence lifetime to condensation state through the mechanical effects that accompany the structural changes and are reflected through altered viscosity. The establishment of FLIM for resolving and quantifying chromatin condensation state opens the door for single-measurement mechanical studies of the nucleus and for characterizing the role of genome structure and organization in nuclear processes that accompany physiological and pathological changes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.