ArticleACS chemical neuroscience2016
Quantification of Histamine and Carcinine in Drosophila melanogaster Tissues.
Article in ACS chemical neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
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- Metabolomic Hallmarks of Obesity and Metabolic Dysfunction-Associated Steatotic Liver Disease.International journal of molecular sciences · 2024Review
- Drosophila mutants lacking the glial neurotransmitter-modifying enzyme Ebony exhibit low neurotransmitter levels and altered behavior.Scientific reports · 2023Article
- Regulation and modulation of biogenic amine neurotransmission inFrontiers in physiology · 2023Review
- Brain histamine and oleoylethanolamide restore behavioral deficits induced by chronic social defeat stress in mice.Neurobiology of stress · 2021Article
- Ring Finger Protein 11 (RNF11) Modulates Dopamine Release in Drosophila.Neuroscience · 2021Article
- Measurement of natural variation of neurotransmitter tissue content in red harvester ant brains among different colonies.Analytical and bioanalytical chemistry · 2020Article
- Dietary yeast influences ethanol sedation in Drosophila via serotonergic neuron function.Addiction biology · 2020Article
- Mechanism of Histamine Oxidation and Electropolymerization at Carbon Electrodes.Analytical chemistry · 2019Article
- Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection.Journal of visualized experiments : JoVE · 2019Article
- Polymer Modified Carbon Fiber-Microelectrodes and Waveform Modifications Enhance Neurotransmitter Metabolite Detection.Analytical methods : advancing methods and applications · 2019Article
- Article
- Drosophila as a Model System for Neurotransmitter Measurements.ACS chemical neuroscience · 2018Review
- Insect ArylalkylamineFrontiers in molecular biosciences · 2018Review
- Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods.Analytical chemistry · 2017Article
- Goggatomy: A Method for Opening Small Cuticular Compartments in Arthropods for Physiological Experiments.Frontiers in physiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Histamine is a neurotransmitter crucial to the visual processing of Drosophila melanogaster. It is inactivated by metabolism to carcinine, a β-alanyl derivative, and the same enzyme that controls that process also converts dopamine to N-β-alanyl-dopamine. Direct detection of histamine and carcinine has not been reported in single Drosophila brains. Here, we quantify histamine, carcinine, dopamine, and N-β-alanyl-dopamine in Drosophila tissues by capillary electrophoresis coupled to fast-scan cyclic voltammetry (CE-FSCV). Limits of detection were low, 4 ± 1 pg for histamine, 10 ± 4 pg for carcinine, 2.8 ± 0.3 pg for dopamine, and 9 ± 3 pg for N-β-alanyl-dopamine. Tissue content was compared in the brain, eyes, and cuticle from wild-type (Canton S) and mutant (tan(3) and ebony(1)) strains. In tan(3) mutants, the enzyme that produces histamine from carcinine is nonfunctional, whereas in ebony(1) mutants, the enzyme that produces carcinine from histamine is nonfunctional. In all fly strains, the neurotransmitter content was highest in the eyes and there were no strain differences for tissue content in the cuticle. The main finding was that carcinine levels changed significantly in the mutant flies, whereas histamine levels did not. In particular, tan(3) flies had significantly higher carcinine levels in the eyes and brain than Canton S or ebony(1) flies. N-β-Alanyl-dopamine was detected in tan(3) mutants but not in other strains. These results show the utility of CE-FSCV for sensitive detection of histamine and carcinine, which allows a better understanding of their content and metabolism in different types of tissues to be obtained.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.