ArticleThe Plant cell2016
Reconstitution of CO2 Regulation of SLAC1 Anion Channel and Function of CO2-Permeable PIP2;1 Aquaporin as CARBONIC ANHYDRASE4 Interactor.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 145 citations in OpenAlex.
- Insights into Salinity Stress-Induced Morpho-Physiological and Molecular Responses and Nanoparticle- and Nanobiochar-Mediated Tolerance Mechanisms During Seed Germination.Nanomaterials (Basel, Switzerland) · 2026Review
- Arbuscular Mycorrhiza Fungi Reduce Photosystem II Efficiency in Phosphorus-Deficient Maize Without Promoting Growth.Plants (Basel, Switzerland) · 2026Article
- Transcriptome Dynamics Provide Insight into the Mechanisms Underlying Cucumber Stomatal Movement Regulated by Blue Light (BL) and Drought Stress.International journal of molecular sciences · 2026Article
- A guard cell carbonic anhydrase binds and regulates SLAC1 separate from its catalytic activity.Nature communications · 2026Article
- Mechanism of COThe Journal of physiology · 2026Article
- Replacement of chromosome 3D with Thinopyrum chromosome 3St led to increased drought tolerance during the flowering stage in wheat.Plant cell reports · 2025Article
- ALMT12 interacts with and inhibits SLAC1 to modulate stomatal movements and enhance plant biomass.Plant physiology · 2025Article
- Amino Acid Substitutions in Loop C of Arabidopsis PIP2 Aquaporins Alters the Permeability of COPlant, cell & environment · 2025Article
- The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure.Journal of experimental botany · 2025Article
- Role of channels in thebioRxiv : the preprint server for biology · 2025Article
- Exploring the Potential of Selenium-Containing Amine (Se-AMA) to Enhance Photosynthesis and Leaf Water Content: New Avenues for Carbonic Anhydrase Modulation inPlants (Basel, Switzerland) · 2025Article
- TOT3-AHA1 module: its role in fine-tuning stomatal responses.Frontiers in plant science · 2025Review
- Stomatal CO2 responses at sub- and above-ambient CO2 levels employ different pathways in Arabidopsis.Plant physiology · 2024Article
- Two aquaporins, PIP1;1 and PIP2;1, mediate the uptake of neonicotinoid pesticides in plants.Plant communications · 2024Article
- A network-based modeling framework reveals the core signal transduction network underlying high carbon dioxide-induced stomatal closure in guard cells.PLoS biology · 2024Article
- Cooperation of an external carbonic anhydrase and HCO3- transporter supports underwater photosynthesis in submerged leaves of the amphibious plant Hygrophila difformis.Annals of botany · 2024Article
- Carbonic anhydrases in the cell wall and plasma membrane ofFrontiers in molecular biosciences · 2024Article
- Methyl viologen-induced changes in the Arabidopsis proteome implicate PATELLIN 4 in oxidative stress responses.Journal of experimental botany · 2024Article
- Cryo-EM structures of the plant anion channel SLAC1 from Arabidopsis thaliana suggest a combined activation model.Nature communications · 2023Article
- OsαCA1 Affects Photosynthesis, Yield Potential, and Water Use Efficiency in Rice.International journal of molecular sciences · 2023Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Dark respiration causes an increase in leaf CO2 concentration (Ci), and the continuing increases in atmospheric [CO2] further increases Ci. Elevated leaf CO2 concentration causes stomatal pores to close. Here, we demonstrate that high intracellular CO2/HCO3 (-) enhances currents mediated by the Arabidopsis thaliana guard cell S-type anion channel SLAC1 upon coexpression of any one of the Arabidopsis protein kinases OST1, CPK6, or CPK23 in Xenopus laevis oocytes. Split-ubiquitin screening identified the PIP2;1 aquaporin as an interactor of the βCA4 carbonic anhydrase, which was confirmed in split luciferase, bimolecular fluorescence complementation, and coimmunoprecipitation experiments. PIP2;1 exhibited CO2 permeability. Mutation of PIP2;1 in planta alone was insufficient to impair CO2- and abscisic acid-induced stomatal closing, likely due to redundancy. Interestingly, coexpression of βCA4 and PIP2;1 with OST1-SLAC1 or CPK6/23-SLAC1 in oocytes enabled extracellular CO2 enhancement of SLAC1 anion channel activity. An inactive PIP2;1 point mutation was identified that abrogated water and CO2 permeability and extracellular CO2 regulation of SLAC1 activity. These findings identify the CO2-permeable PIP2;1 as key interactor of βCA4 and demonstrate functional reconstitution of extracellular CO2 signaling to ion channel regulation upon coexpression of PIP2;1, βCA4, SLAC1, and protein kinases. These data further implicate SLAC1 as a bicarbonate-responsive protein contributing to CO2 regulation of S-type anion channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.