ArticleMolecular biology of the cell2016
Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- C-mannosyl tryptophan dynamics in a mouse model of the peritoneal dissemination of ovarian cancer.The Journal of biological chemistry · 2026Article
- C-mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells.FEBS letters · 2025Article
- C-mannosyltransferase DPY19L1L-mediated Reissner Fiber formation is critical for zebrafish (Science advances · 2025Article
- Identification of C-mannosylation in a receptor tyrosine kinase AXL.Glycobiology · 2024Article
- C-Mannosyl tryptophan is a novel biomarker for thrombocytosis of myeloproliferative neoplasms.Scientific reports · 2024Article
- DPY19L3 promotes vasculogenic mimicry by itsOncology research · 2024Article
- A global view of the human post-translational modification landscape.The Biochemical journal · 2023Review
- Destabilization of vitelline membrane outer layer protein 1 homolog (VMO1) by C-mannosylation.FEBS open bio · 2023Article
- Development of a Machine Learning Model to Predict Non-Durable Response to Anti-TNF Therapy in Crohn's Disease Using Transcriptome Imputed from Genotypes.Journal of personalized medicine · 2022Article
- ErbB4-mediated regulation of vasculogenic mimicry capability in breast cancer cells.Cancer science · 2022Article
- Autoantibodies against cytoskeletons and lysosomal trafficking discriminate sarcoidosis from healthy controls, tuberculosis and lung cancers.Molecular biomedicine · 2022Article
- Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts.Molecules (Basel, Switzerland) · 2021Article
- ProteinMolecules (Basel, Switzerland) · 2021Review
- Yeast- and antibody-based tools for studying tryptophan C-mannosylation.Nature chemical biology · 2021Article
- Quantification of serum C-mannosyl tryptophan by novel assay to evaluate renal function and vascular complications in patients with type 2 diabetes.Scientific reports · 2021Article
- Monomeric C-mannosyl tryptophan is a degradation product of autophagy in cultured cells.Glycoconjugate journal · 2020Article
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- C-Mannosyl tryptophan increases in the plasma of patients with ovarian cancer.Oncology letters · 2020Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
R-spondin1 (Rspo1) is a secreted protein that enhances Wnt signaling, which has crucial functions in embryonic development and several cancers. C-mannosylation is a rare type of glycosylation and might regulate secretion, protein-protein interactions, and enzymatic activity. Although human Rspo1 contains 2 predicted C-mannosylation sites, C-mannosylation of Rspo1 has not been reported, nor have its functional effects on this protein. In this study, we demonstrate by mass spectrometry that Rspo1 is C-mannosylated at W(153) and W(156). Using Lec15.2 cells, which lack dolichol-phosphate-mannose synthesis activity, and mutant Rspo1-expressing cells that replace W(153) and W(156) by alanine residues, we observed that C-mannosylation of Rspo1 is required for its secretion. Further, the enhancement of canonical Wnt signaling by Rspo1 is regulated by C-mannosylation. Recently DPY19 was reported to be a C-mannosyltransferase in Caenorhabditis elegans, but no C-mannosyltransferases have been identified in any other organism. In gain- and loss-of-function experiments, human DPY19L3 selectively modified Rspo1 at W(156) but not W(153) based on mass spectrometry. Moreover, knockdown of DPY19L3 inhibited the secretion of Rspo1. In conclusion, we identified DPY19L3 as the C-mannosyltransferase of Rspo1 at W(156) and found that DPY19L3-mediated C-mannosylation of Rspo1 at W(156) is required for its secretion.
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