ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2016
A case of mistaken identity: are reactive oxygen species actually reactive sulfide species?
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 76 citations in OpenAlex.
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- Transcription factor OxyR regulates sulfane sulfur removal and L-cysteine biosynthesis inApplied and environmental microbiology · 2023Article
- Antioxidant Activity in Extracts fromMolecules (Basel, Switzerland) · 2023Review
- SOD1 is an essential HProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Effects of Antioxidant Nutraceuticals on Cellular Sulfur Metabolism and Signaling.Antioxidants & redox signaling · 2023Review
- WR1065 conjugated to thiol-PEG polymers as novel anticancer prodrugs: broad spectrum efficacy, synergism, and drug resistance reversal.Frontiers in oncology · 2023Article
- Recent Trends and Developments in Multifunctional Nanoparticles for Cancer Theranostics.Molecules (Basel, Switzerland) · 2022Review
- Elemental Sulfur Inhibits Yeast Growth via Producing Toxic Sulfide and Causing Disulfide Stress.Antioxidants (Basel, Switzerland) · 2022Article
- Bad Smells and Broken DNA: A Tale of Sulfur-Nucleic Acid Cooperation.Antioxidants (Basel, Switzerland) · 2021Review
- Green tea polyphenolic antioxidants oxidize hydrogen sulfide to thiosulfate and polysulfides: A possible new mechanism underpinning their biological action.Redox biology · 2020Article
- Reactive Sulfur Species: A New Redox Player in Cardiovascular Pathophysiology.Arteriosclerosis, thrombosis, and vascular biology · 2020Article
- Manganese Porphyrin-Based SOD Mimetics Produce Polysulfides from Hydrogen Sulfide.Antioxidants (Basel, Switzerland) · 2019Article
- OxyR senses sulfane sulfur and activates the genes for its removal in Escherichia coli.Redox biology · 2019Article
- Reactivity of Small Oxoacids of Sulfur.Molecules (Basel, Switzerland) · 2019Review
- Inorganic Polysulfides and Related Reactive Sulfur–Selenium Species from the Perspective of Chemistry.Molecules (Basel, Switzerland) · 2019Review
- Methylene Blue Administration During and After Life-Threatening Intoxication by Hydrogen Sulfide: Efficacy Studies in Adult Sheep and Mechanisms of Action.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Matrix metalloproteinase-2-induced epidermal growth factor receptor transactivation impairs redox balance in vascular smooth muscle cells and facilitates vascular contraction.Redox biology · 2018Article
- Metabolism of hydrogen sulfide (HRedox biology · 2018Article
- The Reactive Species Interactome: Evolutionary Emergence, Biological Significance, and Opportunities for Redox Metabolomics and Personalized Medicine.Antioxidants & redox signaling · 2017Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stepwise one-electron reduction of oxygen to water produces reactive oxygen species (ROS) that are chemically and biochemically similar to reactive sulfide species (RSS) derived from one-electron oxidations of hydrogen sulfide to elemental sulfur. Both ROS and RSS are endogenously generated and signal via protein thiols. Given the similarities between ROS and RSS, we wondered whether extant methods for measuring the former would also detect the latter. Here, we compared ROS to RSS sensitivity of five common ROS methods: redox-sensitive green fluorescent protein (roGFP), 2', 7'-dihydrodichlorofluorescein, MitoSox Red, Amplex Red, and amperometric electrodes. All methods detected RSS and were as, or more, sensitive to RSS than to ROS. roGFP, arguably the "gold standard" for ROS measurement, was more than 200-fold more sensitive to the mixed polysulfide H2Sn(n = 1-8) than to H2O2 These findings suggest that RSS may be far more prevalent in intracellular signaling than previously appreciated and that the contribution of ROS may be overestimated. This conclusion is further supported by the observation that estimated daily sulfur metabolism and ROS production are approximately equal and the fact that both RSS and antioxidant mechanisms have been present since the origin of life, nearly 4 billion years ago, long before the rise in environmental oxygen 600 million years ago. Although ROS are assumed to be the most biologically relevant oxidants, our results question this paradigm. We also anticipate our findings will direct attention toward development of novel and clinically relevant anti-(RSS)-oxidants.
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