Evidence map›Paper›PMID 26762382›Full record

ArticleThe Journal of membrane biology2016

Effects of Phospholipase A2 Inhibitors on Bilayer Lipid Membranes.

Mikhail V Dubinin, Maxim E Astashev, Nikita V Penkov, Sergey V Gudkov, Igor A Dyachenko, Victor N Samartsev, Konstantin N Belosludtsev

Abstract read
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In one paragraph

Article in The Journal of membrane biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Functions and mechanisms of cytosolic phospholipase ANeural regeneration research · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Mikhail V DubininMari State University, pl. Lenina 1, Yoshkar-Ola, Mari El, Russia, 424001. dubinin1989@gmail.com.ORCID 0000-0002-7453-3390
Maxim E AstashevInstitute of Theoretical and Experimental Biophysics RAS, Institutskaya 3, Pushchino, Moscow Region, Russia, 142290.
Nikita V PenkovInstitute of Cell Biophysics RAS, Institutskaya 3, Pushchino, Moscow Region, Russia, 142290.
Sergey V GudkovInstitute of Theoretical and Experimental Biophysics RAS, Institutskaya 3, Pushchino, Moscow Region, Russia, 142290.
Igor A DyachenkoBranch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, pr. Nauki 6, Pushchino, Moscow Region, Russia, 142290.
Victor N SamartsevMari State University, pl. Lenina 1, Yoshkar-Ola, Mari El, Russia, 424001.
Konstantin N BelosludtsevMari State University, pl. Lenina 1, Yoshkar-Ola, Mari El, Russia, 424001.
Mari State University · RUInstitute of Theoretical and Experimental Biophysics · RUInstitute of Bioorganic Chemistry · RUInstitute of Cell Biophysics · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The work examines the effect of inhibitors of cytosolic Ca(2+)-dependent and Ca(2+)-independent phospholipases A2 on bilayer lipid membranes. It was established that trifluoroperazine (TFP) and, to a lesser extent, arachidonyl trifluoromethyl ketone (AACOCF3) and palmitoyl trifluoromethyl ketone (PACOCF3) were able to permeabilize artificial lipid membranes (BLM and liposomes). It was shown that AACOCF3 lowered the temperature of phase transition of DMPC liposomes, inducing disordering of the hydrophobic region of lipid bilayer. TFP disordered membranes both in the hydrophobic region and in the region of hydrophilic heads, this being accompanied by changes in the membrane permeability: appearance of a channel-like BLM activity and leakage of sulforhodamine B from liposomes. In contrast to AACOCF3 and TFP, PACOCF3 increased membrane orderliness in the hydrophobic region (heightened the temperature of phase transition of DMPC liposomes) and in the region of lipid heads. The effectiveness of AACOCF3 and PACOCF3 as inductors of BLM and liposome permeabilization was considerably lower comparatively to TFP. As revealed by dynamic light scattering, incorporation of TFP, AACOCF3 and PACOCF3 into the membrane of liposomes resulted in the increase of the average size of particles in the suspension, presumably due to their aggregation or fusion. The paper discusses possible mechanisms of the influence of phospholipase A2 inhibitors on bilayer lipid membranes.

Indexed as

Cell Membrane PermeabilityElectric ConductivityLipid BilayersLiposomesMembrane FusionMolecular StructurePhosphatidylcholinesPhospholipase A2 InhibitorsPhospholipases A2RhodaminesSpectrometry, FluorescenceTemperatureLipid BilayersLiposomeslissamine rhodamine BPhosphatidylcholinesPhospholipase A2 InhibitorsPhospholipases A2RhodaminesLipid poresLiposomesMembrane fusionMembrane permeabilizationPhase transitionsPhospholipase A2

Identifiers

PMID26762382
OpenAlexW2235240620

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.