ArticleBMC musculoskeletal disorders2016
Molecular changes indicative of cartilage degeneration and osteoarthritis development in patients with anterior cruciate ligament injury.
Article in BMC musculoskeletal disorders, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 39 citations in OpenAlex.
- Mode of injury and level of synovitis alter inflammatory chondrocyte gene expression and associated pathways.Scientific reports · 2024Article
- Effects of alendronate on cartilage lesions and micro-architecture deterioration of subchondral bone in patellofemoral osteoarthritic ovariectomized rats with patella-baja.Journal of orthopaedic surgery and research · 2024Article
- Glucosamine-loaded injectable hydrogel promotes autophagy and inhibits apoptosis after cartilage injury.Heliyon · 2023Article
- Osteoporosis in Light of a New Mechanism Theory of Delayed Onset Muscle Soreness and Non-Contact Anterior Cruciate Ligament Injury.International journal of molecular sciences · 2022Article
- Shear strain and inflammation-induced fixed charge density loss in the knee joint cartilage following ACL injury and reconstruction: A computational study.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Development of new cartilage lesions after ACL reconstruction is associated with abnormal knee rotation.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2022Article
- Articular cartilage and synovium may be important sources of post-surgical synovial fluid inflammatory mediators.American journal of translational research · 2022Article
- Low-Input RNA-Sequencing in Patients with Cartilage Lesions, Osteoarthritis, and Healthy Cartilage.Cartilage · 2021Article
- There is an Association of Synovial Interleukin-6 Levels With Chondral Damage in Anterior Cruciate Ligament-Deficient Knees.HSS journal : the musculoskeletal journal of Hospital for Special Surgery · 2021Article
- Comparison of magnetic resonance imaging for patients with acute and chronic anterior cruciate ligament tears.Medicine · 2018Observational
- Correlations between inflammatory cytokines, muscle damage markers and acute postoperative pain following primary total knee arthroplasty.BMC musculoskeletal disorders · 2017Article
- Articular cartilage status 2 years after arthroscopic ACL reconstruction in patients with or without concomitant meniscal surgery: evaluation with 3.0T MR imaging.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2017Article
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnterior cruciate ligament (ACL) tear is considered a risk factor for osteoarthritis development. The purpose of our study was to investigate the expression levels of the apoptotic enzyme caspase 3, pro-inflammatory cytokines interleukin-1β (IL-1β) and interleukin-6 (IL-6) and degrading enzyme matrix metalloproteinase 13 (MMP-13), all indicative of cartilage degeneration and osteoarthritis development in patients' chondrocytes after ACL rupture.
methodsWe investigated the correlation between grade of cartilage degradation and time from injury or patients' age. IL-1β, IL-6 and MMP-13 mRNA expression levels were investigated in normal (n = 4) and chondrocytes from patients with ACL rupture (n = 33) using real-time polymerase chain reaction (PCR). Moreover, MMP-13 and caspase-3 protein expression levels were evaluated by western blot analysis. Trend analysis and correlation coefficient were performed to derive the relations between gene expression (MMP13, IL-6, IL-1β) and grading of cartilage defects and between gene expression (MMP13, IL-6, IL-1β) and patients' age, respectively.
resultsCorrelations were established between grade of cartilage degradation and time from injury. MMP-13, IL-6, IL-1β and caspase 3 expression levels were significantly upregulated in chondrocytes from ACL-deficient knee compared to normal. Among the patients with ACL-deficient knees, a significant upregulation of MMP-13 was observed in patients with ACL-rupture > 18 months from the time of injury to arthroscopy compared to patients with ACL-injury up to 18 months, whereas IL-6 and IL-1β expression was higher in chondrocytes from patients with more than 10 months ACL injury compared to those that underwent surgery within the first 10 months after injury. Νο association was observed between IL-1β, IL-6 and MMP-13 expression levels and cartilage defects or patients' age.
conclusionOur results showed that increased levels of apoptotic, inflammatory and catabolic factors in chondrocytes are associated with time from injury and could contribute to cartilage degradation and osteoarthritis development after ACL rupture.
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