ArticleKorean journal for food science of animal resources2014
Effect of NaCl on Biofilm Formation of the Isolate from Staphylococcus aureus Outbreak Linked to Ham.
Article in Korean journal for food science of animal resources, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 39 citations in OpenAlex.
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- Characterization of a newly isolated biosurfactant fengycin produced by Heyndrickxia coagulans strain.AMB Express · 2025Article
- Formation of Mono-Organismal and MixedMicroorganisms · 2025Article
- Assessment of antibiofilm and quorum quenching potencies of environmental bacteria in controlling biofilm of food spoilage bacteria.BMC research notes · 2025Article
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- Sub-inhibitory concentrations of ceftriaxone induce morphological alterations and PIA-independent biofilm formation in Staphylococcus aureus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
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- The potential of Bacillus and Enterococcus probiotic strains to combat helicobacter pylori attachment to the biotic and abiotic surfaces.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Virulence Potential of Biofilm-ProducingAntibiotics (Basel, Switzerland) · 2022Article
- Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water.Pathogens (Basel, Switzerland) · 2022Article
- The Response and Survival Mechanisms ofFoods (Basel, Switzerland) · 2022Article
- Effects of NaCl Concentrations on Growth Patterns, Phenotypes Associated With Virulence, and Energy Metabolism inFrontiers in microbiology · 2021Article
- Effect of Sodium Chloride on Pyrite Bioleaching and Initial Attachment byFrontiers in microbiology · 2020Article
- The Uptake and Release of Amino Acids byFrontiers in microbiology · 2019Article
- Combination Susceptibility Testing of Common AntimicrobialsFrontiers in microbiology · 2017Article
- Environmental factors modulate biofilm formation byScience progressReview
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to evaluate the effects of NaCl on the biofilm formations of the isolate from Staphylococcus aureus outbreaks linked to ham. The S. aureus ATCC13565 isolated from ham was exposed to NaCl concentrations of 0%, 2%, 4%, and 6% supplemented in tryptic soy broth (TSB) for 24 h at 35℃, followed by plating 0.1 mL of the culture on tryptic soy agar containing 0%, 2%, 4%, and 6% NaCl, respectively. After incubating at 35℃ for 24 h, the colonies on the plates were collected and diluted to OD600 = 0.1. The diluents of S. aureus were incubated on a 96-well flat bottom plate containing TSB plus the appropriate NaCl concentrations, and the biofilm formation was quantified by crystal violet staining after being incubated at 35℃ for 9 h. Confocal laser scanning microscope (CLSM) was also used for visualizing the biofilm formation of S. aureus at NaCl concentrations of 0%, 2%, 4%, and 6%. The transcriptional analysis of biofilm-related genes, such as icaA, atl, clfA, fnbA, sarA, and rbf, was conducted by quantitative real-time PCR. Crystal violet staining and CLSM showed that the biofilm formations of S. aureus increased (p<0.05) along with the NaCl concentrations. Moreover, the expression of the icaA genes was higher at the NaCl concentrations of 4% and 6% as compared with 0% of NaCl by approximately 9-folds and 20-folds, respectively. These results indicated that the NaCl formulated in processed food may increase the biofilm formations of S. aureus by increasing the icaA gene expressions.
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