ArticleRNA (New York, N.Y.)2016
Cross-backbone templating; ribodinucleotides made on poly(C).
Article in RNA (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 11 citations in OpenAlex.
- Geochemical influences on nonenzymatic oligomerization of prebiotically relevant cyclic nucleotides.RNA (New York, N.Y.) · 2020Article
- Template-Directed Catalysis of a Multistep Reaction Pathway for Nonenzymatic RNA Primer Extension.Biochemistry · 2019Article
- Alarmones as Vestiges of a Bygone RNA World.Journal of molecular evolution · 2019Article
- Eighty routes to a ribonucleotide world; dispersion and stringency in the decisive selection.RNA (New York, N.Y.) · 2018Article
- A Light-Releasable Potentially Prebiotic Nucleotide Activating Agent.Journal of the American Chemical Society · 2018Article
- Non-Watson-Crick RNA synthesis suited to origin functions.RNA (New York, N.Y.) · 2018Article
- A Kinetic Model of Nonenzymatic RNA Polymerization by Cytidine-5'-phosphoro-2-aminoimidazolide.Biochemistry · 2017Article
- Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Efficient Heritable Gene Expression Readily Evolves in RNA Pools.Journal of molecular evolution · 2017Article
- Biochemical Refinement Before Genetics: Chance Utility.Journal of molecular evolution · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G(5')pp(5')G synthesis from pG and chemically activated 2MeImpG is accelerated by the addition of complementary poly(C), but affected only slightly by poly(G) and not at all by poly(U) and poly(A). This suggests that 3'-5' poly(C) is a template for uncatalyzed synthesis of 5'-5' GppG, as was poly(U) for AppA synthesis, previously. The reaction occurs at 50 mM mono- and divalent ion concentrations, at moderate temperatures, and near pH 7. The reactive complex at the site of enhanced synthesis of 5'-5' GppG seems to contain a single pG, a single phosphate-activated nucleotide 2 MeImpG, and a single strand of poly(C). Most likely this structure is base-paired, as the poly(C)-enhanced reaction is completely disrupted between 30 and 37 °C, whereas slower, untemplated synthesis of GppG accelerates. More specifically, the reactive center acts as would be expected for short, isolated G nucleotide stacks expanded and ordered by added poly(C). For example, poly(C)-mediated GppG production is very nonlinear in overall nucleotide concentration. Uncatalyzed NppN synthesis is now known for two polymers and their complementary free nucleotides. These data suggest that varied, simple, primordial 3'-5' RNA sequences could express a specific chemical phenotype by encoding synthesis of complementary, reactive, coenzyme-like 5'-5' ribodinucleotides.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.