ArticleScientific reports2016
Establishment of a novel in vitro model of stratified epithelial wound healing with barrier function.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
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- Development of In Vitro Dry Eye Models to Study Proliferative and Anti-Inflammatory Effects of Allogeneic Serum Eye Drops.International journal of molecular sciences · 2023Article
- Electrospun-Reinforced Suturable Biodegradable Artificial Cornea.ACS applied bio materials · 2022Article
- Crosslinker-free collagen gelation for corneal regeneration.Scientific reports · 2022Article
- Metallic Engineered Nanomaterials and Ocular Toxicity: A Current Perspective.Pharmaceutics · 2022Review
- Graphene-Lined Porous Gelatin Glycidyl Methacrylate Hydrogels: Implications for Tissue Engineering.ACS applied nano materials · 2021Article
- Targeting myosin 1c inhibits murine hepatic fibrogenesis.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization.Burns & trauma · 2021Article
- In silico study reveals binding potential of rotenone at multiple sites of pulmonary surfactant proteins: A matter of concern.Current research in toxicology · 2021Article
- Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye.Pharmaceutics · 2020Review
- Golgi α1,2-mannosidase I induces clustering and compartmentalization of CD147 during epithelial cell migration.Cell adhesion & migration · 2020Article
- Effect of Flightless I Expression on Epidermal Stem Cell Niche During Wound Repair.Advances in wound care · 2020Article
- Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts.Acta biomaterialia · 2019Article
- Probing dynamic processes in the eye at multiple spatial and temporal scales with multimodal full field OCT.Biomedical optics express · 2019Article
- Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea.Journal of visualized experiments : JoVE · 2018Article
- 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Mechanics of epithelial tissues during gap closure.Current opinion in cell biology · 2016Review
- An in vitro 3-dimensional Collagen-based Corneal Construct with Innervation Using Human Corneal Cell Lines.Ophthalmology scienceArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
The repair of wounds through collective movement of epithelial cells is a fundamental process in multicellular organisms. In stratified epithelia such as the cornea and skin, healing occurs in three steps that include a latent, migratory, and reconstruction phases. Several simple and inexpensive assays have been developed to study the biology of cell migration in vitro. However, these assays are mostly based on monolayer systems that fail to reproduce the differentiation processes associated to multilayered systems. Here, we describe a straightforward in vitro wound assay to evaluate the healing and restoration of barrier function in stratified human corneal epithelial cells. In this assay, circular punch injuries lead to the collective migration of the epithelium as coherent sheets. The closure of the wound was associated with the restoration of the transcellular barrier and the re-establishment of apical intercellular junctions. Altogether, this new model of wound healing provides an important research tool to study the mechanisms leading to barrier function in stratified epithelia and may facilitate the development of future therapeutic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.