ArticleBiomedical microdevices2016
A parallelized microfluidic DNA bisulfite conversion module for streamlined methylation analysis.
Article in Biomedical microdevices, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA.Clinical epigenetics · 2021Pooled it
- Lossless Altered Histone Modification Analysis System (LAHMAS).Lab on a chip · 2025Article
- Cancer Methylation Biomarker Detection in an Automated, Portable, Multichannel Magnetofluidic Platform.ACS nano · 2024Article
- High-throughput sample processing for methylation analysis in an automated, enclosed environment.SLAS technology · 2022Article
- Droplet Magnetofluidic Assay Platform for Quantitative Methylation-Specific PCR.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- Microfluidic MeDIP-seq for low-input methylomic analysis of mammary tumorigenesis in mice.The Analyst · 2019Article
- Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation.Scientific reports · 2018Article
- Microfluidic Low-Input Fluidized-Bed Enabled ChIP-seq Device for Automated and Parallel Analysis of Histone Modifications.Analytical chemistry · 2018Article
- A sample-to-answer droplet magnetofluidic assay platform for quantitative methylation-specific PCR.Biomedical microdevices · 2018Article
- Cell-type-specific brain methylomes profiled via ultralow-input microfluidics.Nature biomedical engineering · 2018Article
- Proceedings of the 3rd Annual Albert Institute for Bladder Cancer Research Symposium.Bladder cancer (Amsterdam, Netherlands) · 2017Article
- Microfluidics for genome-wide studies involving next generation sequencing.Biomicrofluidics · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Aberrant methylation of DNA has been identified as an epigenetic biomarker for numerous cancer types. The vast majority of techniques aimed at detecting methylation require bisulfite conversion of the DNA sample prior to analysis, which until now has been a benchtop process. Although microfluidics has potential benefits of simplified operation, sample and reagent economy, and scalability, bisulfite conversion has yet to be implemented in this format. Here, we present a novel droplet microfluidic design that facilitates rapid bisulfite conversion by reducing the necessary processing steps while retaining comparable performance to existing methods. This new format has a reduced overall processing time and is readily scalable for use in high throughput DNA methylation analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.