ArticleScientific reports2016
An innovative platform for quick and flexible joining of assorted DNA fragments.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- The plant Golden Gate toolkit: compatibility, interoperability, and harmonized assembly in plant synthetic biology.Plant methods · 2026Review
- UniClo technology exploits methylation for universal scarless DNA assembly.Biology methods & protocols · 2026Review
- DASH: a versatile and high-capacity gene stacking system for plant synthetic biology.Plant biotechnology journal · 2025Review
- UniClo: scarless hierarchical DNA assembly without sequence constraint.Nucleic acids research · 2025Article
- Article
- A User's Guide to Golden Gate Cloning Methods and Standards.ACS synthetic biology · 2022Review
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
- Biosystems Design to Accelerate CBiodesign research · 2020Review
- A high-throughput transient expression system for rice.Plant, cell & environment · 2019Article
- Standardizing Automated DNA Assembly: Best Practices, Metrics, and Protocols Using Robots.SLAS technology · 2019Article
- MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme.Nucleic acids research · 2018Article
- The Kalanchoë genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism.Nature communications · 2017Article
- GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data.Nucleic acids research · 2017Article
- Editorial: Plant Molecular Farming: Fast, Scalable, Cheap, Sustainable.Frontiers in plant science · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Successful synthetic biology efforts rely on conceptual and experimental designs in combination with testing of multi-gene constructs. Despite recent progresses, several limitations still hinder the ability to flexibly assemble and collectively share different types of DNA segments. Here, we describe an advanced system for joining DNA fragments from a universal library that automatically maintains open reading frames (ORFs) and does not require linkers, adaptors, sequence homology, amplification or mutation (domestication) of fragments in order to work properly. This system, which is enhanced by a unique buffer formulation, provides unforeseen capabilities for testing, and sharing, complex multi-gene circuitry assembled from different DNA fragments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.