Evidence map›Paper›PMID 26758460›Full record

ArticleJournal of assisted reproduction and genetics2016

Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles.

Keewan Kim, Michael S Bloom, Victor Y Fujimoto, Erin M Bell, Recai M Yucel, Richard W Browne

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Keewan KimDepartment of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA.
Michael S BloomDepartment of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA. mbloom@albany.edu.ORCID http://orcid.org/0000-0002-0028-5494
Victor Y FujimotoDepartment of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA, USA.
Erin M BellDepartment of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA.
Recai M YucelDepartment of Epidemiology and Biostatistics, University at Albany, State University of New York, Rensselaer, NY, USA.
Richard W BrowneDepartment of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, Buffalo, NY, USA.
Albany State University · USUniversity at Albany, State University of New York · USUniversity at Buffalo, State University of New York · USUniversity of California, San Francisco · US

Funding

Developmental Infrastructure CoreR24HD044943 · NICHD · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI GAGE, TIMOTHY B · 2004 to 2016
$4.8M
High Density Lipoprotein (HDL) in Follicular Fluid and Fertility OutcomesR21AG031957 · NIA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FUJIMOTO, VICTOR YUTAKA · 2010 to 2011
$443k
NIA NIH HHS R21 AG031957NIA NIH HHS R21 AG031957-01A2NICHD NIH HHS R24 HD044943
6 · The paper itself

Abstract

purposeThe purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles.

methodsWe collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography. We assessed biological variability using two-stage nested analysis of variance and compared values with those previously published for contralateral follicles.

resultsFor most FF HDL analytes, there was little variability between follicles relative to the variability between women (i.e., %σ(2) F:%σ(2) B <0.5). Intraclass correlation coefficients were >0.80 for HDL cholesterol (0.82), phospholipids (0.89), paraoxonase (0.96), and arylesterase (0.91) activities, ApoA-1 (0.89), and ApoA-2 (0.90), and single specimen collections were required to estimate the subject-specific mean, demonstrating sufficient reliability for use as biomarkers of the follicular microenvironment in epidemiologic and clinical studies.

conclusionsThese preliminary results raise the possibility for tighter regulation of HDL in follicles within the same ovary vs. between ovaries. Thus, collection of a single FF specimen may be sufficient to estimate HDL particle components concentrations within a single ovary. However, our results should be interpreted with caution as the analysis was based on a small sample.

Indexed as

AdultFemaleFertilization in VitroFollicular FluidHumansLipoproteins, HDLOvarian FollicleLipoproteins, HDLBiological variabilityFollicular fluid (FF)High density lipoprotein (HDL)Human ovarian folliclesIn vitro fertilization (IVF)

Identifiers

PMID26758460
PMCPMC4785162
OpenAlexW2238775983

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.