ArticleNature communications2016
A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 75 citations in OpenAlex.
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- Functional unknomics: Systematic screening of conserved genes of unknown function.PLoS biology · 2023Article
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- The effect of Drosophila attP40 background on the glomerular organization of Or47b olfactory receptor neurons.G3 (Bethesda, Md.) · 2023Article
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- A genetic screen in Drosophila reveals the role of fucosylation in host susceptibility to Candida infection.Disease models & mechanisms · 2022Article
- Genome-wide phenotypic RNAi screen in the Drosophila wing: global parameters.G3 (Bethesda, Md.) · 2021Article
- Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.G3 (Bethesda, Md.) · 2021Article
- Cp1/cathepsin L is required for autolysosomal clearance inAutophagy · 2021Article
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Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Libraries of transgenic Drosophila melanogaster carrying RNA interference (RNAi) constructs have been used extensively to perform large-scale functional genetic screens in vivo. For example, RNAi screens have facilitated the discovery of multiple components of the Hippo pathway, an evolutionarily conserved growth-regulatory network. Here we investigate an important technical limitation with the widely used VDRC KK RNAi collection. We find that approximately 25% of VDRC KK RNAi lines cause false-positive enhancement of the Hippo pathway, owing to ectopic expression of the Tiptop transcription factor. Of relevance to the broader Drosophila community, ectopic tiptop (tio) expression can also cause organ malformations and mask phenotypes such as organ overgrowth. To enhance the use of the VDRC KK RNAi library, we have generated a D. melanogaster strain that will allow researchers to test, in a single cross, whether their genetic screen of interest will be affected by ectopic tio expression.
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