ArticleBMC cell biology2016
The role of neural connexins in HeLa cell mobility and intercellular communication through tunneling tubes.
Article in BMC cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Article
- Disconnecting multicellular networks in brain tumours.Nature reviews. Cancer · 2022Review
- The Network of Tumor Microtubes: An Improperly Reactivated Neural Cell Network With Stemness Feature for Resistance and Recurrence in Gliomas.Frontiers in oncology · 2022Review
- Membrane nanotubes are ancient machinery for cell-to-cell communication and transport. Their interference with the immune system.Biologia futura · 2021Review
- ELAVL1 Role in Cell Fusion and Tunneling Membrane Nanotube Formations with Implication to Treat Glioma Heterogeneity.Cancers · 2020Review
- Tunneling Nanotubes and Tumor Microtubes in Cancer.Cancers · 2020Review
- Tunneling nanotubes: A bridge for heterogeneity in glioblastoma and a new therapeutic target?Cancer reports (Hoboken, N.J.) · 2019Review
- Midbody: From the Regulator of Cytokinesis to Postmitotic Signaling Organelle.Medicina (Kaunas, Lithuania) · 2018Review
- Signaling pathways regulating blood-tissue barriers - Lesson from the testis.Biochimica et biophysica acta. Biomembranes · 2018Review
- Gap junctional signaling in pattern regulation: Physiological network connectivity instructs growth and form.Developmental neurobiology · 2017Review
- Connexins in the Central Nervous System: Physiological Traits and Neuroprotective Targets.Frontiers in physiology · 2017Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMembranous tunneling tubes (TTs) are a recently discovered new form of communication between remote cells allowing their electrical synchronization, migration, and transfer of cellular materials. TTs have been identified in the brain and share similarities with neuronal processes. TTs can be open-ended, close-ended or contain functional gap junctions at the membrane interface. Gap junctions are formed of two unapposed hemichannels composed of six connexin (Cx) subunits. There are evidences that Cxs also play channel-independent role in cell adhesion, migration, division, differentiation, formation of neuronal networks and tumorigenicity. These properties of Cxs and TTs may synergetically determine the cellular and intercellular processes. Therefore, we examined the impact of Cxs expressed in the nervous system (Cx36, Cx40, Cx43, Cx45, and Cx47) on: 1) cell mobility; 2) formation and properties of TTs; and 3) transfer of siRNA between remote cells through TTs.
resultsWe have identified two types of TTs between HeLa cells: F-actin rich only and containing F-actin and α-tubulin. The morphology of TTs was not influenced by expression of examined connexins; however, Cx36-EGFP-expressing cells formed more TTs while cells expressing Cx43-EGFP, Cx45, and Cx47 formed fewer TTs between each other compared with wt and Cx40-CFP-expressing cells. Also, Cx36-EGFP and Cx40-CFP-expressing HeLa cells were more mobile compared with wt and other Cxs-expressing cells. TTs containing Cx40-CFP, Cx43-EGFP, or Cx47 gap junctions were capable of transmitting double-stranded small interfering RNA; however, Cx36-EGFP and Cx45 were not permeable to it. In addition, we show that Cx43-EGFP-expressing HeLa cells and laryngeal squamous cell carcinoma cells can couple to the mesenchymal stem cells through TTs.
conclusionsDifferent Cxs may modulate the mobility of cells and formation of TTs in an opposite manner; siRNA transfer through the GJ-containing TTs is Cx isoform-dependent.
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