ArticleMicrobial cell factories2016
Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline cellulose.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 122 citations in OpenAlex.
- Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.Biotechnology for biofuels and bioproducts · 2026Review
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing.Microbial biotechnology · 2023Review
- Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from COMicrobial cell factories · 2022Article
- De novo biosynthesis of p-coumaric acid and caffeic acid from carboxymethyl-cellulose by microbial co-culture strategy.Microbial cell factories · 2022Article
- Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels.Bioengineered · 2022Review
- Consolidated Bioprocessing: Synthetic Biology Routes to Fuels and Fine Chemicals.Microorganisms · 2021Review
- Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges.Biotechnology for biofuels · 2021Review
- Recent progress on bio-succinic acid production from lignocellulosic biomass.World journal of microbiology & biotechnology · 2021Review
- Developing Clostridia as Cell Factories for Short- and Medium-Chain Ester Production.Frontiers in bioengineering and biotechnology · 2021Review
- The production of isoprene from cellulose using recombinant Clostridium cellulolyticum strains expressing isoprene synthase.MicrobiologyOpen · 2020Article
- Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review.Microbial cell factories · 2020Review
- Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization.Microbial biotechnology · 2020Review
- Metabolic and evolutionary responses ofBiotechnology for biofuels · 2020Article
- Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis.Microbial cell factories · 2019Article
- Improved electrocompetence and metabolic engineering ofEngineering in life sciences · 2019Article
- ImprovedApplied and environmental microbiology · 2019Article
- Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacteriumBiotechnology for biofuels · 2019Article
- Metabolic engineering ofBiotechnology for biofuels · 2019Article
- Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.Biotechnology for biofuels · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSustainable alternatives for the production of fuels and chemicals are needed to reduce our dependency on fossil resources and to avoid the negative impact of their excessive use on the global climate. Lignocellulosic feedstock from agricultural residues, energy crops and municipal solid waste provides an abundant and carbon-neutral alternative, but it is recalcitrant towards microbial degradation and must therefore undergo extensive pretreatment to release the monomeric sugar units used by biofuel-producing microbes. These pretreatment steps can be reduced by using microbes such as Clostridium cellulolyticum that naturally digest lignocellulose, but this limits the range of biofuels that can be produced. We therefore developed a metabolic engineering approach in C. cellulolyticum to expand its natural product spectrum and to fine tune the engineered metabolic pathways.
resultsHere we report the metabolic engineering of C. cellulolyticum to produce n-butanol, a next-generation biofuel and important chemical feedstock, directly from crystalline cellulose. We introduced the CoA-dependent pathway for n-butanol synthesis from C. acetobutylicum and measured the expression of functional enzymes (using targeted proteomics) and the abundance of metabolic intermediates (by LC-MS/MS) to identify potential bottlenecks in the n-butanol biosynthesis pathway. We achieved yields of 40 and 120 mg/L n-butanol from cellobiose and crystalline cellulose, respectively, after cultivating the bacteria for 6 and 20 days.
conclusionThe analysis of enzyme activities and key intracellular metabolites provides a robust framework to determine the metabolic flux through heterologous pathways in C. cellulolyticum, allowing further improvements by fine tuning individual steps to improve the yields of n-butanol.
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