ArticleScientific reports2016
Two-Photon Excitation STED Microscopy with Time-Gated Detection.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Super-resolution imaging using fluorescence-lifetime emission-difference.Biomedical optics express · 2026Article
- Wavelength-tunable depletion expands fluorophore compatibility in two-photon STED nanoscopy.Biomedical optics express · 2026Article
- Correlative multiphoton-STED microscopy of podocyte calcium levels and slit diaphragm ultrastructure in the renal glomerulus.Scientific reports · 2024Article
- Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology.Plant biotechnology journal · 2023Review
- Article
- Multiphoton single-molecule localization by sequential excitation with light minima.Light, science & applications · 2022Article
- Commercially derived versatile optical architecture for two-photon STED, wavelength mixing and label-free microscopy.Biomedical optics express · 2022Article
- Efficient two-photon excitation stimulated emission depletion nanoscope exploiting spatiotemporal information.Neurophotonics · 2019Article
- Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses.Biomedical optics express · 2019Article
- Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles.Nature communications · 2018Article
- Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts.Biomedical optics express · 2018Article
- Coherent optical adaptive technique improves the spatial resolution of STED microscopy in thick samples.Photonics research · 2017Article
- Power Flow in a Large-Core Multimode Fiber under External Perturbation and its Applications.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on a novel two-photon excitation stimulated emission depletion (2PE-STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the fluorophores using moderate stimulated emission beam intensity. This opens the possibility of implementing an efficient 2PE-STED microscope with a stimulated emission beam running in a continuous-wave. The continuous-wave stimulated emission beam tempers the laser architecture's complexity and cost, but the time-gated detection degrades the signal-to-noise ratio (SNR) and signal-to-background ratio (SBR) of the image. We recover the SNR and the SBR through a multi-image deconvolution algorithm. Indeed, the algorithm simultaneously reassigns early-photons (normally discarded by the time-gated detection) to their original positions and removes the background induced by the stimulated emission beam. We exemplify the benefits of this implementation by imaging sub-cellular structures. Finally, we discuss of the extension of this algorithm to future all-pulsed 2PE-STED implementationd based on time-gated detection and a nanosecond laser source.
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