ArticleMolecular plant pathology2016
A novel sweet potato potyvirus open reading frame (ORF) is expressed via polymerase slippage and suppresses RNA silencing.
Article in Molecular plant pathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- Evaluating the Negative-Strand Coding Potential in Plum Pox Virus.Molecular plant pathology · 2025Article
- Development of convenient potyvirus-based vectors for simultaneous expression of two heterologous proteins in solanaceous plants.BMC plant biology · 2025Article
- Complete genome sequence and molecular characterization of a novel potyvirus (sweet potato virus H) infecting sweet potato.Archives of virology · 2025Article
- A conserved lysine/arginine-rich motif is essential for the autophagic degradation of potyviral 6K1 protein and virus infection.Journal of virology · 2025Article
- Next Generation Sequencing and Genetic Analyses Reveal Factors Driving Evolution of Sweetpotato Viruses in Uganda.Pathogens (Basel, Switzerland) · 2024Article
- Article
- The 6-kilodalton peptide 1 in plant viruses of the family Potyviridae is a viroporin.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Quantitative Analysis of RNA Polymerase Slippages for Production of P3N-PIPO Trans-frame Fusion Proteins in Potyvirids.Journal of microbiology (Seoul, Korea) · 2023Article
- CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host.Communications biology · 2023Article
- A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus.Journal of virology · 2023Article
- P1 of turnip mosaic virus interacts with NOD19 for vigorous infection.Frontiers in microbiology · 2023Article
- AlkB RNA demethylase homologues and NMolecular plant pathology · 2022Article
- Article
- Proteome expansion in the Potyviridae evolutionary radiation.FEMS microbiology reviews · 2022Review
- An Importin-β-like Protein fromViruses · 2021Article
- Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of P3N-PIPO in a Bymovirus.Viruses · 2021Article
- P1 ofJournal of virology · 2021Article
- The P1 Protein ofFrontiers in microbiology · 2021Article
- A Newly Identified Virus in the FamilyJournal of virology · 2020Article
- Functional Characterization of Pepper Vein Banding Virus-Encoded Proteins and Their Interactions: Implications inViruses · 2020Review
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Authors and funding
6 authors at 3 institutions in 4 countries.
Funding
Abstract
The single-stranded, positive-sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Four related potyviruses infecting sweet potato (Ipomoea batatas) are predicted to contain a third ORF, called pispo, which overlaps the 3' third of the P1 region. Recently, pipo has been shown to be expressed via polymerase slippage at a conserved GA6 sequence. Here, we show that pispo is also expressed via polymerase slippage at a GA6 sequence, with higher slippage efficiency (∼5%) than at the pipo site (∼1%). Transient expression of recombinant P1 or the 'transframe' product, P1N-PISPO, in Nicotiana benthamiana suppressed local RNA silencing (RNAi), but only P1N-PISPO inhibited short-distance movement of the silencing signal. These results reveal that polymerase slippage in potyviruses is not limited to pipo expression, but can be co-opted for the evolution and expression of further novel gene products.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.