ArticlePhysiological reports2016
Orai1 forms a signal complex with BKCa channel in mesenteric artery smooth muscle cells.
Article in Physiological reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Potassium channels in vascular smooth muscle cells.Physiological reviews · 2026Review
- Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice.Acta pharmacologica Sinica · 2024Article
- Orai1 Boosts SK3 Channel Activation.Cancers · 2021Article
- More Than Just Simple Interaction between STIM and Orai Proteins: CRAC Channel Function Enabled by a Network of Interactions with Regulatory Proteins.International journal of molecular sciences · 2021Review
- Enhanced Store-Operated CaFrontiers in physiology · 2021Article
- Molecular Choreography and Structure of CaMembranes · 2020Review
- TRPP2 and STIM1 form a microdomain to regulate store-operated CaCell communication and signaling : CCS · 2020Article
- Article
- TRPP2 associates with STIM1 to regulate cerebral vasoconstriction and enhance high salt intake-induced hypertensive cerebrovascular spasm.Hypertension research : official journal of the Japanese Society of Hypertension · 2019Article
- Cell Cycle-Dependent Expression of Bk Channels in Human Mesenchymal Endometrial Stem Cells.Scientific reports · 2019Article
- Exercise restores impaired endothelium-derived hyperpolarizing factor-mediated vasodilation in aged rat aortic arteries via the TRPV4-KClinical interventions in aging · 2019Article
- STIM Proteins and Orai CaFrontiers in pharmacology · 2018Article
- Constitutive calcium entry and cancer: updated views and insights.European biophysics journal : EBJ · 2017Review
- Orai1 forms a signal complex with BKCa channel in mesenteric artery smooth muscle cells.Physiological reports · 2016Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Orai1, a specific nonvoltage-gated Ca(2+) channel, has been found to be one of key molecules involved in store-operated Ca(2+) entry (SOCE). Orai1 may associate with other proteins to form a signaling complex, which is essential for regulating a variety of physiological functions. In this study, we studied the possible interaction between Orai1 and large conductance Ca(2+)-activated potassium channel (BKC a). Using RNA interference technique, we demonstrated that the SOCE and its associated membrane hyperpolarization were markedly suppressed after knockdown of Orai1 with a specific Orai1 siRNA in rat mesenteric artery smooth muscle. Moreover, isometric tension measurements showed that agonist-induced vasocontraction was increased after Orai1 was knocked down or the tissue was incubated with BKC a blocker iberiotoxin. Coimmunoprecipitation data revealed that BKC a and Orai1 could reciprocally pull down each other. In situ proximity ligation assay further demonstrated that Orai1 and BKC a are in close proximity. Taken together, these results indicate that Orai1 physically associates with BKC a to form a signaling complex in the rat mesenteric artery smooth muscle. Ca(2+) influx via Orai1 stimulates BKC a, leading to membrane hyperpolarization. This hyperpolarizing effect of Orai1-BKC a coupling could contribute to reduce agonist-induced membrane depolarization, therefore preventing excessive contraction of the rat mesenteric artery smooth muscle in response to contractile agonists.
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