ArticleThe Journal of biological chemistry2016
Regulatory Characteristics of Vibrio vulnificus gbpA Gene Encoding a Mucin-binding Protein Essential for Pathogenesis.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Fe-S cluster homeostasis and beyond: The multifaceted roles of IscR.Biochimica et biophysica acta. Molecular cell research · 2024Review
- Tools to Enumerate and Predict Distribution Patterns of EnvironmentalMicroorganisms · 2023Review
- Modeling pH and Temperature Effects as Climatic Hazards in VGeoHealth · 2023Article
- Environmental Reservoirs of Pathogenic Vibrio spp. and Their Role in Disease: The List Keeps Expanding.Advances in experimental medicine and biology · 2023Review
- Two novel genes identified by large-scale transcriptomic analysis are essential for biofilm and rugose colony development of Vibrio vulnificus.PLoS pathogens · 2023Article
- Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis.BMC microbiology · 2022Article
- Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus.NPJ biofilms and microbiomes · 2021Article
- A Nitric Oxide-Responsive Transcriptional Regulator NsrR Cooperates With Lrp and CRP to Tightly Control theFrontiers in microbiology · 2021Article
- A MARTX ToxinmBio · 2020Article
- The transcriptional regulator IscR integrates host-derived nitrosative stress and iron starvation in activation of theThe Journal of biological chemistry · 2020Article
- Melatonin restores Muc2 depletion induced by V. vulnificus VvpM via melatonin receptor 2 coupling with Gαq.Journal of biomedical science · 2020Article
- Small-molecule inhibitor of HlyU attenuates virulence of Vibrio species.Scientific reports · 2019Article
- A Novel Tetrameric Assembly Configuration in VV2_1132, a LysR-Type Transcriptional Regulator inMolecules and cells · 2018Article
- Crystal structure of peroxiredoxin 3 fromIUCrJ · 2018Article
- Article
- Identification and characterization ofThe Journal of biological chemistry · 2017Article
- Crystal Structure of the Regulatory Domain of AphB fromMolecules and cells · 2017Article
- Vibrio vulnificus: From Oyster Colonist to Human Pathogen.PLoS pathogens · 2017Review
- AFrontiers in cellular and infection microbiology · 2017Article
- Effects of BlueM® against Streptococcus mutans biofilm and its virulence gene expression.Brazilian dental journalArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Binding to mucin is the initial step for enteropathogens to establish pathogenesis. An open reading frame, gbpA, of Vibrio vulnificus was identified and characterized in this study. Compared with wild type, the gbpA mutant was impaired in binding to mucin-agar and the mucin-secreting HT29-methotrexate cells, and the impaired mucin binding was restored by the purified GbpA provided exogenously. The gbpA mutant had attenuated virulence and ability of intestinal colonization in a mouse model, indicating that GbpA is a mucin-binding protein and essential for pathogenesis of V. vulnificus. The gbpA transcription was growth phase-dependent, reaching a maximum during the exponential phase. The Fe-S cluster regulator (IscR) and the cyclic AMP receptor protein (CRP) coactivated, whereas SmcR, a LuxR homologue, repressed gbpA. The cellular levels of IscR, CRP, and SmcR were not significantly affected by one another, indicating that the regulator proteins function cooperatively to regulate gbpA rather than sequentially in a regulatory cascade. The regulatory proteins directly bind upstream of the gbpA promoter PgbpA. DNase I protection assays, together with the deletion analyses of PgbpA, demonstrated that IscR binds to two specific sequences centered at -164.5 and -106, and CRP and SmcR bind specifically to the sequences centered at -68 and -45, respectively. Furthermore, gbpA was induced by exposure to H2O2, and the induction appeared to be mediated by elevated intracellular levels of IscR. Consequently, the combined results indicated that IscR, CRP, and SmcR cooperate for precise regulation of gbpA during the V. vulnificus pathogenesis.
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