ArticleProceedings of the National Academy of Sciences of the United States of America2016
Developmental accumulation of inorganic polyphosphate affects germination and energetic metabolism in Dictyostelium discoideum.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 56 citations in OpenAlex.
- Probing metazoan polyphosphate biology usingeLife · 2026Article
- Article
- Platelet Polyphosphate Signals Through NFκB to Induce Myofibroblast Differentiation.Biomolecules · 2025Article
- Two endogenousProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enabling Technologies for the Dissection of Inositol Pyrophosphate Physiology.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A fusion protein of polyphosphate kinase 1 (PPK1) and a Nudix hydrolase is involved in inorganic polyphosphate accumulation in the unicellular red alga Cyanidioschyzon merolae.Plant molecular biology · 2024Article
- A mammalian model reveals inorganic polyphosphate channeling into the nucleolus and induction of a hyper-condensate state.Cell reports methods · 2024Article
- The Protein Scaffolding Functions of Polyphosphate.Journal of molecular biology · 2024Review
- Evolutionary perspective on mammalian inorganic polyphosphate (polyP) biology.Biochemical Society transactions · 2023Review
- Starvation Induces Extracellular Accumulation of Polyphosphate inInternational journal of molecular sciences · 2023Article
- Dictyostelium discoideum cells retain nutrients when the cells are about to outgrow their food source.Journal of cell science · 2022Article
- Effects of Polyphosphate on Leukocyte Function.Progress in molecular and subcellular biology · 2022Review
- The inositol pyrophosphate metabolism of Dictyostelium discoideum does not regulate inorganic polyphosphate (polyP) synthesis.Advances in biological regulation · 2022Article
- The PPIP5K Family Member Asp1 Controls Inorganic Polyphosphate Metabolism inJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Model systems for studying polyphosphate biology: a focus on microorganisms.Current genetics · 2021Review
- Depletion of mitochondrial inorganic polyphosphate (polyP) in mammalian cells causes metabolic shift from oxidative phosphorylation to glycolysis.The Biochemical journal · 2021Article
- Transcriptional Responses ofFrontiers in microbiology · 2021Article
- Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation.Journal of thrombosis and haemostasis : JTH · 2020Article
- Inorganic polyphosphate controls cyclophilin B-mediated collagen folding in osteoblast-like cells.The FEBS journal · 2020Article
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3 authors at 3 institutions in 1 country.
Funding
Abstract
Inorganic polyphosphate (polyP) is composed of linear chains of phosphate groups linked by high-energy phosphoanhydride bonds. However, this simple, ubiquitous molecule remains poorly understood. The use of nonstandardized analytical methods has contributed to this lack of clarity. By using improved polyacrylamide gel electrophoresis we were able to visualize polyP extracted from Dictyostelium discoideum. We established that polyP is undetectable in cells lacking the polyphosphate kinase (DdPpk1). Generation of this ppk1 null strain revealed that polyP is important for the general fitness of the amoebae with the mutant strain displaying a substantial growth defect. We discovered an unprecedented accumulation of polyP during the developmental program, with polyP increasing more than 100-fold. The failure of ppk1 spores to accumulate polyP results in a germination defect. These phenotypes are underpinned by the ability of polyP to regulate basic energetic metabolism, demonstrated by a 2.5-fold decrease in the level of ATP in vegetative ppk1. Finally, the lack of polyP during the development of ppk1 mutant cells is partially offset by an increase of both ATP and inositol pyrophosphates, evidence for a model in which there is a functional interplay between inositol pyrophosphates, ATP, and polyP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.