ArticleScientific reports2016
Ammonia-induced miRNA expression changes in cultured rat astrocytes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Effect of Per and Poly-Fluoroalkyl Substances on Pregnancy and Child Development.Current pediatric reviews · 2025Review
- Unraveling the Role of miR-200b-3p in Attention-Deficit/Hyperactivity Disorder (ADHD) and Its Therapeutic Potential in Spontaneously Hypertensive Rats (SHR).Biomedicines · 2024Article
- Cellular Pathogenesis of Hepatic Encephalopathy: An Update.Biomolecules · 2023Review
- Hepatic encephalopathy.Nature reviews. Disease primers · 2022Review
- Diagnosis and Therapeutic Management of Liver Fibrosis by MicroRNA.International journal of molecular sciences · 2021Review
- Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.Disease models & mechanisms · 2020Article
- Ammonia-Induced Glial-Inflammaging.Molecular neurobiology · 2020Article
- MicroRNAs and Xenobiotic Toxicity: An Overview.Toxicology reports · 2020Review
- Xiaochaihutang Improves the Cortical Astrocyte Edema in Thioacetamide-Induced Rat Acute Hepatic Encephalopathy by Activating NRF2 Pathway.Frontiers in pharmacology · 2020Article
- Analysis of Risk Factors for Postoperative Delirium After Liver Transplantation.Neuropsychiatric disease and treatment · 2020Article
- Hepatic encephalopathy is linked to alterations of autophagic flux in astrocytes.EBioMedicine · 2019Article
- Hepatic Encephalopathy and Astrocyte Senescence.Journal of clinical and experimental hepatology · 2018Review
- Suppression of miR-26a attenuates physiological disturbances arising from exposure of Nile tilapia (Biology open · 2018Article
- Ammonia Attenuates LPS-Induced Upregulation of Pro-Inflammatory Cytokine mRNA in Co-Cultured Astrocytes and Microglia.Neurochemical research · 2017Article
- Recent advances in hepatic encephalopathy.F1000Research · 2017Review
- In low protein diets, microRNA-19b regulates urea synthesis by targeting SIRT5.Scientific reports · 2016Article
- MiRNAs in Astrocyte-Derived Exosomes as Possible Mediators of Neuronal Plasticity.Journal of experimental neuroscience · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatic encephalopathy is a neuropsychiatric syndrome evolving from cerebral osmotic disturbances and oxidative/nitrosative stress. Ammonia, the main toxin of hepatic encephalopathy, triggers astrocyte senescence in an oxidative stress-dependent way. As miRNAs are critically involved in cell cycle regulation and their expression may be regulated by oxidative stress, we analysed, whether astrocyte senescence is a consequence of ammonia-induced miRNA expression changes. Using a combined miRNA and gene microarray approach, 43 miRNA species which were downregulated and 142 genes which were upregulated by NH4Cl (5 mmol/l, 48 h) in cultured rat astrocytes were found. Ammonia-induced miRNA and gene expression changes were validated by qPCR and 43 potential miRNA target genes, including HO-1, were identified by matching upregulated mRNA species with predicted targets of miRNA species downregulated by ammonia. Inhibition of HO-1 targeting miRNAs which were downregulated by NH4Cl strongly upregulated HO-1 mRNA and protein levels and inhibited astrocyte proliferation in a HO-1-dependent way. Preventing ammonia-induced upregulation of HO-1 by taurine (5 mmol/l) as well as blocking HO-1 activity by tin-protoporphyrine IX fully prevented ammonia-induced proliferation inhibition and senescence. The data suggest that ammonia induces astrocyte senescence through NADPH oxidase-dependent downregulation of HO-1 targeting miRNAs and concomitant upregulation of HO-1 at both mRNA and protein level.
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