ArticleInfection and immunity2016
Nonselective Persistence of a Rickettsia conorii Extrachromosomal Plasmid during Mammalian Infection.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- DRP1/DMNL-1-mediated mitochondrial fission augmentsInfection and immunity · 2026Article
- Recent advances in genetic systems in obligate intracellular human-pathogenic bacteria.Frontiers in cellular and infection microbiology · 2023Review
- Procedure for spotted fever group Rickettsia isolation from limited clinical blood specimens.PLoS neglected tropical diseases · 2022Article
- Examination of Rickettsial Host Range for Shuttle Vectors Based onApplied and environmental microbiology · 2022Article
- Spotted Fever GroupMicrobiology spectrum · 2021Article
- Rickettsia conorii survival in THP-1 macrophages involves host lipid droplet alterations and active rickettsial protein production.Cellular microbiology · 2021Article
- Isolate-Dependent Differences in Clinical, Pathological, and Transcriptional Profiles followingInfection and immunity · 2021Article
- Significant Growth byPathogens (Basel, Switzerland) · 2021Article
- The guinea pig model for tick-borne spotted fever rickettsioses: A second look.Ticks and tick-borne diseases · 2020Review
- Recent research milestones in the pathogenesis of human rickettsioses and opportunities ahead.Future microbiology · 2020Review
- Evasion of autophagy mediated by Rickettsia surface protein OmpB is critical for virulence.Nature microbiology · 2019Article
- Macrophages Infected by a Pathogen and a Non-pathogen Spotted Fever GroupFrontiers in cellular and infection microbiology · 2019Article
- A Pathogen and a Non-pathogen Spotted Fever GroupFrontiers in cellular and infection microbiology · 2019Article
- Immunity against the Obligate Intracellular Bacterial Pathogen Rickettsia australis Requires a Functional Complement System.Infection and immunity · 2018Article
- Engineering of obligate intracellular bacteria: progress, challenges and paradigms.Nature reviews. Microbiology · 2017Review
- GFPuv-Expressing Recombinant Rickettsia typhi: a Useful Tool for the Study of Pathogenesis and CD8Infection and immunity · 2017Article
- TheFrontiers in cellular and infection microbiology · 2017Article
- Effect of Amblyomma maculatum (Acari: Ixodidae) Saliva on the Acute Cutaneous Immune Response to Rickettsia parkeri Infection in a Murine Model.Journal of medical entomology · 2016Article
- Differences in Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells.Frontiers in cellular and infection microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Scientific analysis of the genus Rickettsia is undergoing a rapid period of change with the emergence of viable genetic tools. The development of these tools for the mutagenesis of pathogenic bacteria will permit forward genetic analysis of Rickettsia pathogenesis. Despite these advances, uncertainty still remains regarding the use of plasmids to study these bacteria in in vivo mammalian models of infection, namely, the potential for virulence changes associated with the presence of extrachromosomal DNA and nonselective persistence of plasmids in mammalian models of infection. Here, we describe the transformation of Rickettsia conorii Malish 7 with the plasmid pRam18dRGA[AmTrCh]. Transformed R. conorii stably maintains this plasmid in infected cell cultures, expresses the encoded fluorescent proteins, and exhibits growth kinetics in cell culture similar to those of nontransformed R. conorii. Using a well-established murine model of fatal Mediterranean spotted fever, we demonstrate that R. conorii(pRam18dRGA[AmTrCh]) elicits the same fatal outcomes in animals as its untransformed counterpart and, importantly, maintains the plasmid throughout infection in the absence of selective antibiotic pressure. Interestingly, plasmid-transformed R. conorii was readily observed both in endothelial cells and within circulating leukocytes. Together, our data demonstrate that the presence of an extrachromosomal DNA element in a pathogenic rickettsial species does not affect either in vitro proliferation or in vivo infectivity in models of disease and that plasmids such as pRam18dRGA[AmTrCh] are valuable tools for the further genetic manipulation of pathogenic rickettsiae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.