Evidence map›Paper›PMID 26754671›Full record

ArticleWorld journal of microbiology & biotechnology2016

Ultrastructural effects and antibiofilm activity of LFchimera against Burkholderia pseudomallei.

Aekkalak Puknun, Sakawrat Kanthawong, Chitchanok Anutrakunchai, Kamran Nazmi, Wikky Tigchelaar, Kees A Hoeben, Enno C I Veerman, Jan G M Bolscher, Suwimol Taweechaisupapong

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. LFchimera protects HeLa cells from invasion by Yersinia spp. in vitro.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2018
    Article
  6. Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Aekkalak PuknunBiofilm Research Group, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand.
Sakawrat KanthawongBiofilm Research Group, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand.
Chitchanok AnutrakunchaiBiofilm Research Group, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand.
Kamran NazmiDepartment of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.
Wikky TigchelaarCore Facility Cellular Imaging/LCAM-AMC, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands.
Kees A HoebenCore Facility Cellular Imaging/LCAM-AMC, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands.
Enno C I VeermanDepartment of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.
Jan G M BolscherDepartment of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.
Suwimol TaweechaisupapongBiofilm Research Group, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand. suvi_taw@kku.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lactoferrin chimera (LFchimera), a hybrid peptide containing the two antimicrobial stretches of the innate immunity factor bovine lactoferrin, viz. LFampin265-284 and LFcin17-30, has strikingly high antimicrobial activity against the category B pathogen Burkholderia pseudomallei. The action mechanisms of LFchimera against B. pseudomallei is not fully understood. The aim of this study was to further investigate the effect of treated B. pseudomallei with LFchimera using (immune) electron microscopy. The effects of LFchimera on biofilm formation and against preformed biofilm of B. pseudomallei were also determined. After exposure to LFchimera, transmission electron microscopy revealed swelling of the periplasmic space of B. pseudomallei and a highly inhomogeneous electron density in the intracellular DNA region. Localization of LFchimera in B. pseudomallei using immunoelectron microscopy showed gold particles in intracellular structures without accumulation on the membranes. LFchimera also possessed stronger bactericidal activity than ceftazidime against B. pseudomallei grown in biofilm. Moreover, limited exposure of B. pseudomallei to LFchimera at subcidal concentration could reduce biofilm formation. Altogether, the results indicate that LFchimera possesses antibacterial and antibiofilm activities and can modulate B. pseudomallei colonization. Therefore, the efficacy of LFchimera merits further development of this agent for the therapy of melioidosis.

Indexed as

Amino Acid SequenceAnimalsAnti-Bacterial AgentsAntimicrobial Cationic PeptidesBiofilmsBurkholderia pseudomalleiCattleCeftazidimeCell MembraneMelioidosisMicrobial Sensitivity TestsMicroscopy, ElectronAnti-Bacterial AgentsAntimicrobial Cationic PeptidesCeftazidimeLFchimera peptideAntimicrobial peptideBiofilmBurkholderia pseudomallei(Immune) electron microscopyLFchimeraMelioidosis

Identifiers

PMID26754671

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.