Evidence map›Paper›PMID 26754668›Full record

ArticlePlant physiology2016

Analysis of the Rice ADP-Glucose Transporter (OsBT1) Indicates the Presence of Regulatory Processes in the Amyloplast Stroma That Control ADP-Glucose Flux into Starch.

Bilal Cakir, Shota Shiraishi, Aytug Tuncel, Hiroaki Matsusaka, Ryosuke Satoh, Salvinder Singh, Naoko Crofts, Yuko Hosaka, Naoko Fujita, Seon-Kap Hwang and 2 more

Open access · bronzeAbstract read
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In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
17.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 63 citations in OpenAlex.

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  10. Identifying QTLs for Grain Size in a Colossal Grain Rice (International journal of molecular sciences · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Bilal CakirInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).ORCID http://orcid.org/0000-0003-4610-0317
Shota ShiraishiInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).
Aytug TuncelInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).ORCID http://orcid.org/0000-0002-4666-5679
Hiroaki MatsusakaInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).
Ryosuke SatohInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).ORCID http://orcid.org/0000-0003-1439-412X
Salvinder SinghInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).
Naoko CroftsInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).ORCID http://orcid.org/0000-0002-6792-2232
Yuko HosakaInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).
Naoko FujitaInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).ORCID http://orcid.org/0000-0002-6792-2232
Seon-Kap HwangInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.).ORCID http://orcid.org/0000-0001-9552-5341
Hikaru SatohInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.) okita@wsu.edu hikaru.satoh.682@m.kyushu-u.ac.jp.
Thomas W OkitaInstitute of Biological Chemistry, Washington State University, Pullman, Washington 99164 (B.C., A.T., S.-K.H., T.W.O.);Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (S.Sh., H.M., R.S., H.S.);Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785013, Assam, India (S.Si.); andDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-01195, Japan (N.C., Y.H., N.F.) okita@wsu.edu hikaru.satoh.682@m.kyushu-u.ac.jp.ORCID http://orcid.org/0000-0002-2246-0599
Kyushu University · JPWashington State University · USAkita Prefectural University · JPAssam Agricultural University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previous studies showed that efforts to further elevate starch synthesis in rice (Oryza sativa) seeds overproducing ADP-glucose (ADPglc) were prevented by processes downstream of ADPglc synthesis. Here, we identified the major ADPglc transporter by studying the shrunken3 locus of the EM1093 rice line, which harbors a mutation in the BRITTLE1 (BT1) adenylate transporter (OsBt1) gene. Despite containing elevated ADPglc levels (approximately 10-fold) compared with the wild-type, EM1093 grains are small and shriveled due to the reduction in the amounts and size of starch granules. Increases in ADPglc levels in EM1093 were due to their poor uptake of ADP-[(14)C]glc by amyloplasts. To assess the potential role of BT1 as a rate-determining step in starch biosynthesis, the maize ZmBt1 gene was overexpressed in the wild-type and the GlgC (CS8) transgenic line expressing a bacterial glgC-TM gene. ADPglc transport assays indicated that transgenic lines expressing ZmBT1 alone or combined with GlgC exhibited higher rates of transport (approximately 2-fold), with the GlgC (CS8) and GlgC/ZmBT1 (CS8/AT5) lines showing elevated ADPglc levels in amyloplasts. These increases, however, did not lead to further enhancement in seed weights even when these plant lines were grown under elevated CO2. Overall, our results indicate that rice lines with enhanced ADPglc synthesis and import into amyloplasts reveal additional barriers within the stroma that restrict maximum carbon flow into starch.

Indexed as

Adenosine Diphosphate GlucoseGenes, PlantGlucose Transport Proteins, FacilitativeMutationOryzaPlant ProteinsPlants, Genetically ModifiedPlastidsRecombinant ProteinsSeedsStarchZea maysAdenosine Diphosphate GlucoseGlucose Transport Proteins, FacilitativePlant ProteinsRecombinant ProteinsStarch

Identifiers

PMID26754668
PMCPMC4775147
OpenAlexW2237480497

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.