ArticleScientific reports2016
Cryopreservation of the gorgonian endosymbiont Symbiodinium.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 33 citations in OpenAlex.
- Influence of Habitat Alteration on the Molecular Profile of Membrane Lipids of the CoralBiology · 2026Article
- Identification of housekeeping gene for future studies exploring effects of cryopreservation on gene expression in shrimp.Scientific reports · 2025Article
- The Octocoral Trait Database: a global database of trait information for octocoral species.Scientific data · 2025Article
- Article
- Effect of Cryopreservation on Proteins from the Ubiquitous Marine DinoflagellatePlants (Basel, Switzerland) · 2021Article
- First instance of settlement by cryopreserved coral larvae in symbiotic association with dinoflagellates.Scientific reports · 2019Article
- The effects of aquarium culture on coral oocyte ultrastructure.Scientific reports · 2018Article
- Validation of reference genes for cryopreservation studies with the gorgonian coral endosymbiont Symbiodinium.Scientific reports · 2017Article
- Comparison of the cryo-tolerance of vitrified gorgonian oocytes.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study focused on finding a suitable cryoprotectant (CPA) and an optimum freezing protocol for the cryopreservation of the endosymbiotic dinoflagellates (Symbiodinium, clade G) of Junceella fragilis wherein the success of experiments is crucial to both scientific and ecology studies. A two-step freezing technique was developed. The viability of the thawed dinoflagellates was assayed using the adenosine triphosphate (ATP) bioassay for the first time and was further confirmed through the culturing of dinoflagellates in vitro. The results suggested that 30 min was the most suitable holding time for the dinoflagellates, and the samples produced highest viability when suspended at 5 cm from the surface of LN2. Results also showed that 1 M methanol with 0.4 M sucrose was the most effective CPA, yielding the highest viability (56.93%). Although cell densities of both cryopreserved and control group suffered an initial decline of culture, the cell densities were maintained throughout the remaining duration. In the present study, the cryopreservation of clade G endosymbiont algae was studied for the first time and the method described here could be applied for future studies on symbiotic algae cryopreservation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.