ArticleBMC plant biology2016
Cell wall proteome of sugarcane stems: comparison of a destructive and a non-destructive extraction method showed differences in glycoside hydrolases and peroxidases.
Article in BMC plant biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- SsPit2A/B Effectors fromPlants (Basel, Switzerland) · 2026Article
- Characterization and in silico analysis of the domain unknown function DUF568-containing gene family in rice (Oryza sativa L.).BMC genomics · 2023Article
- Plant Cell Wall Proteomes: The Core of Conserved Protein Families and the Case of Non-Canonical Proteins.International journal of molecular sciences · 2022Review
- Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics.BMC plant biology · 2021Article
- Applying Molecular Phenotyping Tools to Explore Sugarcane Carbon Potential.Frontiers in plant science · 2021Review
- Plant Proteomics and Systems Biology.Advances in experimental medicine and biology · 2021Article
- Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development.International journal of molecular sciences · 2019Article
- Sugarcane Omics: An Update on the Current Status of Research and Crop Improvement.Plants (Basel, Switzerland) · 2019Review
- Article
- Plant Cell Wall Proteomics: A Focus on Monocot Species,International journal of molecular sciences · 2019Review
- Plant lipid transfer proteins: are we finally closing in on the roles of these enigmatic proteins?Journal of lipid research · 2018Review
- Transcriptome and secretome analysis of Aspergillus fumigatus in the presence of sugarcane bagasse.BMC genomics · 2018Article
- The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes.Proteomes · 2018Article
- Proteomics Coupled with Metabolite and Cell Wall Profiling Reveal Metabolic Processes of a Developing Rice Stem Internode.Frontiers in plant science · 2017Article
- Review
- Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSugarcane has been used as the main crop for ethanol production for more than 40 years in Brazil. Recently, the production of bioethanol from bagasse and straw, also called second generation (2G) ethanol, became a reality with the first commercial plants started in the USA and Brazil. However, the industrial processes still need to be improved to generate a low cost fuel. One possibility is the remodeling of cell walls, by means of genetic improvement or transgenesis, in order to make the bagasse more accessible to hydrolytic enzymes. We aimed at characterizing the cell wall proteome of young sugarcane culms, to identify proteins involved in cell wall biogenesis. Proteins were extracted from the cell walls of 2-month-old culms using two protocols, non-destructive by vacuum infiltration vs destructive. The proteins were identified by mass spectrometry and bioinformatics.
resultsA predicted signal peptide was found in 84 different proteins, called cell wall proteins (CWPs). As expected, the non-destructive method showed a lower percentage of proteins predicted to be intracellular than the destructive one (33% vs 44%). About 19% of CWPs were identified with both methods, whilst the infiltration protocol could lead to the identification of 75% more CWPs. In both cases, the most populated protein functional classes were those of proteins related to lipid metabolism and oxido-reductases. Curiously, a single glycoside hydrolase (GH) was identified using the non-destructive method whereas 10 GHs were found with the destructive one. Quantitative data analysis allowed the identification of the most abundant proteins.
conclusionsThe results highlighted the importance of using different protocols to extract proteins from cell walls to expand the coverage of the cell wall proteome. Ten GHs were indicated as possible targets for further studies in order to obtain cell walls less recalcitrant to deconstruction. Therefore, this work contributed to two goals: enlarge the coverage of the sugarcane cell wall proteome, and provide target proteins that could be used in future research to facilitate 2G ethanol production.
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